We narrowed to 15,599 results for: LEA

1. GFP-DroshaS302A

   Plasmid

   #62527

   Purpose

   Serine 302 of Drosha is mutated to alanine

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serine 302 changed to alanine (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serine 302 to alanine

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

2. PD-1-miSFIT-1-A, 5-A

   Plasmid

   #124682

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCin4-3xFLAG-MKL1 L272A

   Plasmid

   #19850

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 L272A (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Leucine 272 to Alanine

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

4. NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75233

   Purpose

   CRISPR/Cas9 plasmid against human NFATc2

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCin4-3xFLAG-MKL1 D444-630

   Plasmid

   #19840

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 D444-630 (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 444-630

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5 FRT TO myc Separase delta 55/S1126A

   Plasmid

   #59825

   Purpose

   Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   delta 55/S1126A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCin4-3xFLAG-MKL1 S449A

   Plasmid

   #19842

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 449 to Alanine

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

8. HOXB9 (murine) HIS-tag pET

   Plasmid

   #8541

   Depositor

   Corey Largman

   Article

   Chang et al Mol Cell Biol 1996 Apr;16(4):1734-45.

   Insert

   HOXB9 (Hoxb9 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Missing the first 13 amino acids of HOXB9; makes …

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pBacPak-HA-Non-stop

   Plasmid

   #52289

   Purpose

   Expresses Drosophila Non-stop

   Depositor

   Jerry Workman

   Article

   Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

   Insert

   Non-stop (not Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. sgGCAT_2

    Plasmid

    #72885

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGCAT
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMGF170

    Plasmid

    #96997

    Purpose

    ish1-mCherry insertion cassette with NAT resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + NAT r…

    Promoter

    N/A

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBiex PKC - Peptide 14 mCit mCer

    Plasmid

    #83565

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. MDH1-miR-221SM-PGK-GFP-Blast

    Plasmid

    #31520

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-221 (Mir221 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

14. MDH1-miR-196a-1SM-PGK-GFP-Blast

    Plasmid

    #31510

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a1 (Mir196a-1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

15. Hoxa4-3'UTR

    Plasmid

    #31524

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    Hoxa4-3'UTR (Hoxa4 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pCVL.SSA TLR (Ani)

    Plasmid

    #45577

    Purpose

    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Ani I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pClneoMyc MOB1 T35A

    Plasmid

    #47937

    Purpose

    expresses Myc tagged human MOB1 containing T35A mutation

    Depositor

    Yutaka Hata

    Article

    Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

    Insert

    MOB1A (MOB1A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T35A

    Promoter

    CMV

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

18. MDH1-miR-324SM-PGK-GFP-Blast

    Plasmid

    #31518

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-324 (Mir324 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

19. 3704 pGEX4T-3 MLL AD L2854A

    Plasmid

    #8876

    Depositor

    Stanley Korsmeyer

    Article

    Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

    Insert

    MLL min A.D. (KMT2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    L2854A: ILPSDIMDFVL --> ILPSDIMDFVA

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pET-28a_VIM-2

    Plasmid

    #242840

    Purpose

    Expresses His6-TEV-VIM-2 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-2 sequence (UniPROT Q5U7L7)

    Depositor

    Richard Page

    Article

    Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do

    Insert

    Metallo-beta-lactamase VIM-2
    

    Use

    Tags

    His6 + Tobacco etch virus (TEV) cleavage site “EN…

    Expression

    Bacterial

    Mutation

    deleted residues 1-26

    Promoter

    T7

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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