We narrowed to 9,184 results for: Pol;

1. Frt-V5-hspB1

   Plasmid

   #63102

   Purpose

   Expression of HSPB1 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb1 (HSPB1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

   Plasmid

   #159107

   Purpose

   The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

   Depositor

   Thomas Tuschl

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   NSP12
   

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

   Plasmid

   #216478

   Purpose

   lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   YAP5SA
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Mutation

   S61A, S109A, S127A, S164A, S381A

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGM234

   Plasmid

   #220177

   Purpose

   Expresses exogenous wild-type NAC complex members (NACA and BTF3)

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   NACA and BTF3 (NACA, BTF3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG (NACA); 6xHis (BTF3)

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2273

   Plasmid

   #111899

   Purpose

   ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple343 (TUBB3 Human)
   

   Use

   AAV

   Promoter

   Ple343 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

   Plasmid

   #111389

   Purpose

   AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   ChETA-mRuby2
   W3SL
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   W3SL is built from a shortened WPRE, an upstream …

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EF1α-BHLHE40-IRES-ZsGreen1

   Plasmid

   #153061

   Purpose

   Lentiviral vector expressing human BHLHE40

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   BHLHE40 (BHLHE40 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-Duet-1-NONO(53-312)

   Plasmid

   #135442

   Purpose

   Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   Non-POU domain-containing octamer-binding protein (NONO Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1+/-FLI1-C‐(K)‐DYK

    Plasmid

    #129497

    Purpose

    Mammalian expression of human FLI1

    Depositor

    Marjorie Brand

    Article

    Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

    Insert

    FLI1 (FLI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPRv2_sgCtrl#1

    Plasmid

    #174148

    Purpose

    Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid

    #118586

    Purpose

    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

    Depositor

    Radhakrishnan Padmanabhan

    Article

    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

    Insert

    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    

    Use

    Yeast-e. col shuttle vector prs424

    Mutation

    The conserved GDD in the NS5 polymerase gene muta…

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PH-Btk-mRuby2

    Plasmid

    #124657

    Purpose

    PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    BTK (BTK Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    contains only the PH domain of Bruton tyrosine ki…

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_6xHis-PARP1deltaART

    Plasmid

    #174804

    Purpose

    Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1deltaART (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 785-1014

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Trim46

    Plasmid

    #176402

    Purpose

    TRIM46 tagged with GFP

    Depositor

    Casper Hoogenraad, Thomas Schwarz

    Article

    van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

    Insert

    trim46 (Trim46 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG426GAL-SLEV_NS2B-GS-NS3

    Plasmid

    #203510

    Purpose

    Expresses SLEV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    poly SLEV NS2B-GS-NS3 (POLY )
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_6xHis-PARP1delZn2delBRCT

    Plasmid

    #173939

    Purpose

    Bacterial expression of PARP1 lacking Zn2 and BRCT domains

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn2delBRCT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 97-213 and 374-520; V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS2280

    Plasmid

    #111901

    Purpose

    ssAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple345 (NEFL Human)
    

    Use

    AAV

    Promoter

    Ple345 (NEFL MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Trim46

    Plasmid

    #176401

    Purpose

    TRIM46 tagged with mCherry

    Depositor

    Casper Hoogenraad, Thomas Schwarz

    Article

    van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

    Insert

    trim46 (Trim46 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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