We narrowed to 9,742 results for: Tec

Showing: 6501 - 6520 of 9742 results

Results per page:

Sort results by:

pMEH60 Lamp2b MS2 HA

Plasmid

#168209

Purpose

Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Lamp2b (LAMP2 Human)

Tags

HA and MS2 dimer

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TOMM20-mTurquoise-RhoA-G14V-deltaCaaX

Plasmid

#176123

Purpose

Constitutively active, mitochondrial localized RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOA (RHOA Human)

Expression

Mammalian

Mutation

G14V

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
6xHis-MBP-huPdCas12a

Plasmid

#174682

Purpose

Expresses 6xHis-MBP tagged PdCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPdCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
6xHis-MBP-huPcCas12a

Plasmid

#174681

Purpose

Expresses 6xHis-MBP tagged PcCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPcCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp13 deltaN1-EGFP

Plasmid

#165127

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp13 deltaN2-EGFP

Plasmid

#165128

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUE1-TSP-ORF68 R443A

Plasmid

#162652

Purpose

Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 ZFAND3-guide4

Plasmid

#125516

Purpose

CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 EGFP-guide2

Plasmid

#118167

Purpose

CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIDS-LSD1-C

Plasmid

#109160

Purpose

Encodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)

Expression

Insect

Promoter

p10

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-hPMCA2xb

Plasmid

#47751

Purpose

mammalian expression of mCherry tagged hPMCA2, xb splice variant

Depositor

Emanuel Strehler

Article

Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

Insert

PMCA2x/b (ATP2B2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

xb splice variant

Promoter

CMV

Available Since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only
GFP-ERK2 del174-177

Plasmid

#118297

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 del174-177 (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

deletion 174aa-177aa

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS_N_3xFLAG

Plasmid

#101654

Purpose

Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, 3xFLAG tag

Tags

3xFLAG tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBT9-hSyntenin2b

Plasmid

#89422

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Tags

GAL4 DBD

Expression

Yeast

Mutation

* see below

Promoter

ADH1

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmEos2-Linker_PCNA-MutC

Plasmid

#98272

Purpose

for low level expression of mEos2-PCNA in mammalian cells

Depositor

Mike Heilemann

Article

Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

Insert

PCNA (PCNA Human)

Tags

mEos2

Expression

Mammalian

Mutation

Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSOD1E133K-AcGFP1

Plasmid

#83442

Purpose

Expresses SOD1E133K in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

E133K

Promoter

CMV

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
shMEK1/2 neo

Plasmid

#72569

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-DEST42-ARL3

Plasmid

#67418

Purpose

Expression of hArl3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL6-no stop

Plasmid

#67329

Purpose

hArl6 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only