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We narrowed to 6,999 results for: crispr cas9 plasmids

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Showing: 6501 - 6520 of 6999 results

  2. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid
    #131312
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    131312-plasmid-map-sequence-id-254654

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  8. A3A-PBE-NG

    Plasmid
    #138141
    Purpose
    Targeted C-to-T in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-nCas9-NG-UGI-NLS
    Expression
    Plant
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138141-plasmid-map-sequence-id-311740

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  9. STEME-1

    Plasmid
    #138137
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    Expression
    Plant
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    138137-plasmid-map-sequence-id-320280

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  10. STEME-2

    Plasmid
    #138138
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-APOBEC3A-nCas9-UGI-NLS
    Expression
    Plant
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138138-plasmid-map-sequence-id-311722

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  11. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114073-plasmid-map-sequence-id-220529

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  12. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid
    #114075
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114075-plasmid-map-sequence-id-220517

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  13. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  15. STEME-4

    Plasmid
    #138140
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-APOBEC3A-nCas9-2X(T2A-UGI-NLS)
    Expression
    Plant
    Available Since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    138140-plasmid-map-sequence-id-341208

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  16. STEME-3

    Plasmid
    #138139
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-2X(T2A-UGI-NLS)
    Expression
    Plant
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138139-plasmid-map-sequence-id-311725

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Showing: 6501 - 6520 of 6999 results