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pET21-MRPS2

Plasmid

#31342

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein S2 mature form (MRPS2 Human)

Tags

His

Expression

Bacterial

Mutation

Encodes the predicted mature form for incorporati…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 mu

Plasmid

#44938

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 mu (GRF9 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pANY3

Plasmid

#112197

Purpose

This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-Y-GECO2m

Plasmid

#105070

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in mammalian cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Tags

6x His tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-PHYHIP

Plasmid

#31578

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

phytanoyl-CoA hydroxylase interacting protein (PHYHIP Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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BacPAK GST-Uch37N

Plasmid

#19421

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 1-239 (UCHL5 Human)

Tags

GST and His

Expression

Insect

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pJ414 - DANCER-3

Plasmid

#136387

Purpose

Expresses DANCER-3 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-3

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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LacD_WT_pET21a

Plasmid

#29785

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L165E_pET21a

Plasmid

#29786

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L165E

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L275S_pET21a

Plasmid

#29787

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L275S

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L165E/L275S_pET21a

Plasmid

#29788

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L165E and L275S

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CTNNB1

Plasmid

#67119

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CTNNB1 (CTNNB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pQTEV-PHYHIPL

Plasmid

#34823

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

phytanoyl-CoA hydroxylase interacting protein-like (PHYHIPL Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF3

Plasmid

#67110

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF3 (TCF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

Truncated isoform

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-GAP-BoCCS-GRM

Plasmid

#123286

Purpose

Expression of Glo3 ΔAmpH (1-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (773-908)

Plasmid

#1316

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (773-908) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

deletion of aa1-773

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (361-548)

Plasmid

#1317

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (361-548) (HSP104 Budding Yeast)

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa361-548 of Hsp104

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2.2s

Plasmid

#105069

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.2s

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-MGC20398

Plasmid

#34825

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

MGC20398 (ZNF830 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_wt_Venus_173

Plasmid

#39815

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
BacPAK HF-Uch37

Plasmid

#19425

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 (UCHL5 Human)

Tags

Flag and His

Expression

Insect

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_105_Venus_229

Plasmid

#39822

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
PGRP-LCa

Plasmid

#21059

Depositor

Johann Deisenhofer

Article

Chang et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

PGRP-LCa (PGRP-LF Fly)

Tags

His

Expression

Insect

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pK RagA-RagC(Q120L)

Plasmid

#99701

Purpose

Bacterial expression of RagA-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

Q120L

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter cel-mir-40

Plasmid

#46739

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-40

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Promoter

Metallothionein (MT)

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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PGRP-LCx

Plasmid

#21058

Depositor

Johann Deisenhofer

Article

Chang et al Science. 2006 Mar 24. 311(5768):1761-4.

Insert

PGRP-LCx (PGRP-LC Fly)

Tags

His

Expression

Insect

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
BacPAK GST-Uch37

Plasmid

#19710

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 (UCHL5 Human)

Tags

GST and His

Expression

Insect

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
BacPAK GST-Uch37C

Plasmid

#19422

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 239-329 (UCHL5 Human)

Tags

GST and His

Expression

Insect

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only