We narrowed to 14,200 results for: cas9

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pUFlip-floxed2A-mRFP-; HE1A:BFP -0

Plasmid

#180007

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HMEJ donor

Plasmid

#97318

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_DNMT3B

Plasmid

#72366

Purpose

Encodes gRNA for 3' target of human DNMT3B along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

DNMT3B (DNMT3B Human)

Use

CRISPR

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pJAT27

Plasmid

#204308

Purpose

For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPc

Use

CRISPR

Promoter

MHC

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT18

Plasmid

#204307

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPn

Use

CRISPR

Promoter

MHC

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS269

Plasmid

#73727

Purpose

SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDM067

Plasmid

#216820

Purpose

Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Publication

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mStayGold2

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTPH413

Plasmid

#185728

Purpose

Bacterial PAM profiling of adenine base editor variants

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Inserts

ecTadA(8e)-neNme2-C
Nme2Cas9 sgRNA

Use

CRISPR

Expression

Bacterial

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE WASF1-GFP KI

Plasmid

#131502

Purpose

Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Wasf1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE CACNG2-GFP KI

Plasmid

#131504

Purpose

Endogenous tagging of Tarpγ2/Stargazin: Intramolecular (amino acid position: S265)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng2, Cacng2 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1163

Plasmid

#111099

Purpose

Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCD2 gRNA (FANCD2 Human)

Use

Lentiviral

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST-HemmarG2

Plasmid

#112814

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

Depositor

Chun Han

Publication

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB5791

Plasmid

#106126

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB6633

Plasmid

#106162

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB6627

Plasmid

#106159

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB4666

Plasmid

#106144

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCfB6574

Plasmid

#106132

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PAX6 sgRNA7

Plasmid

#68466

Purpose

targeting PAX6 gene

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

sgRNA targeting PAX6 (PAX6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugABE-NNG-P2A-puro

Plasmid

#214354

Purpose

Expresses SlugABE-NNG and puromycin resistance genes

Depositor

Yongming Wang

Publication

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG and puromycin resistance genes

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Publication

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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