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pBa.Halo-KIF3B tail 391-747

Plasmid

#193929

Purpose

Mammalian expression of Halo-tagged Kif 3B tail 391-747

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3B (Kif3b Mouse)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2C-ΔC

Plasmid

#200555

Purpose

Mammalian expression of secreted mouse CES2C

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-VC155

Plasmid

#194046

Purpose

Transient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VC155 (mVenus β-strands 8 to 11, amino acids 155−239)

Tags

AtOEP7 1–50aa

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KAP3B

Plasmid

#193940

Purpose

Mammalian expression of Halo-tagged KAP 3B

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

KAP 3B (KIFAP3 Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3A tail 419-702

Plasmid

#193923

Purpose

Mammalian expression of GFP-tagged Kif 3A tail 419-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-mCCK2R-stop

Plasmid

#179321

Purpose

Express mouse cholecystokinin 2 receptor (untagged) in mammalian cells

Depositor

Christian Gruber

Article

Taghizadeh et al Sci Rep. 2022 Jun 2;12(1):9215. doi: 10.

Insert

mouse cholecystokinin2 receptor

Expression

Mammalian

Mutation

stop codon after ORF

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)

Plasmid

#185309

Purpose

For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I_47-300

Expression

Mammalian

Mutation

D125I

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT2Aneo-mRFP-FKBP-mSos1-linkercat

Plasmid

#173858

Purpose

Encoding a part of the rapamycin-induced Ras activation system.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

mRFP-FKBP-mSos1-linkercat

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-neo-dTgfa

Plasmid

#173842

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisHbegf

Plasmid

#173846

Purpose

Encoding dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Hbegf.

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisTgfa

Plasmid

#173847

Purpose

Encoding dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEreg

Plasmid

#173848

Purpose

Encoding dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTEF1-UDH

Plasmid

#166679

Purpose

Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

UDH

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_CBX1_Chromo-shadow

Plasmid

#109965

Purpose

Protein expression and purification of CBX1_Chromo-shadow

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CBX1_Chromo-shadow (CBX1 Human)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_IGLL1_C1-set

Plasmid

#109991

Purpose

Protein expression and purification of IGLL1_C1-set

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

IGLL1_C1-set (IGLL1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_KARS_tRNA-anti-codon

Plasmid

#109865

Purpose

Protein expression and purification of KARS_tRNA-anti-codon

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KARS_tRNA-anti-codon (KARS1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYL6_EF-hand

Plasmid

#109794

Purpose

Protein expression and purification of MYL6_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

MYL6_EF-hand (MYL6 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-OMACS

Plasmid

#136765

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Acsm4

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Acsm4 (Acsm4 Mouse)

Use

In situ probe

Mutation

ISH Probe insert, A102T- please see depositor com…

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140170

Purpose

Expresses a regulatable secretory cargo for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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