172,885 results
-
Plasmid#80795PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotypeDepositorTypeEmpty backboneExpressionMammalianPromoterhCMV IE1 gene promoterAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only
-
qTAG-AAVS1-Ef1a-Puro
Plasmid#227266PurposeEmpty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-(-)-Pur
Plasmid#200458PurposeEukaryotic expression vectorDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T4
Plasmid#201767PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
ExpressionYeastAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D1cpV
Plasmid#37479DepositorInsertD1cpV second generation cameleon (calcium sensor)
ExpressionMammalianPromoterCMVAvailable SinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cre-IRES-PuroR
Plasmid#30205PurposeLentiviral coexpression of Cre and puromycin from the human EF1a promoterDepositorInsertCre recombinase (cre Enterobacteria phage P1)
UseCre/Lox and LentiviralExpressionMammalianPromoterEF1alphaAvailable SinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pABY-c46
Plasmid#201774PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
Tags40aa Linker, mNeonGreenExpressionYeastPromoterTEF1Available SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1
Plasmid#187458PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-DJ8R
Plasmid#213974PurposeAAV packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid coding sequenceDepositorTypeEmpty backboneUseAAVAvailable SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only