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pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGV15 (CK2alpha K68M)

Plasmid

#27089

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha K68M (kinase dead) (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Mutation

K68M (kinase dead)

Promoter

CMV

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA-CasRx_SD1-pSV40-TagBFP

Plasmid

#221004

Purpose

Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

CasRx 30nt processed direct repeat with SD1 mutation

Use

CRISPR

Expression

Mammalian

Mutation

DR1 A>T

Promoter

U6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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sfGFP-C1

Plasmid

#54579

Purpose

Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

Depositor

Michael Davidson, Geoffrey Waldo

Article

Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

Type

Empty backbone

Tags

sfGFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD33-Gm

Plasmid

#65098

Purpose

bacterial expression vector with Gentamycin selection, based on pBAD33

Depositor

Juan Tomas

Article

Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

Plasmid

#121538

Purpose

An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

Depositor

Christopher Richie

Article

Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

Has Service

AAV2 and AAV5

Insert

HA-tagged hM4D(Gi) (CHRM4 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

SYN1

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

Plasmid

#154786

Purpose

Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

Depositor

Robert Singer

Article

Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

Insert

Adenosine deaminse (Adar Fly, Synthetic)

Use

Lentiviral

Tags

GFP, V5, and mCherry

Expression

Mammalian

Mutation

Synonymized serine to prevent autoediting of ADAR…

Promoter

UBC

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pZR060_CROPseq-sgCtrl-CRISPRi

Plasmid

#180269

Purpose

Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5B*-strep

Plasmid

#120164

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XHA-EGFP-PEX26

Plasmid

#139054

Purpose

HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XHA-EGFP-PEX26

Use

Lentiviral

Tags

HA

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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mito-CIBN-GFP

Plasmid

#89480

Purpose

Encodes for a fusion protein between a mitochondrial anchor and the CIBN-GFP protein.

Depositor

Xavier Trepat Guixer

Article

Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

Insert

mito-CIBN-GFP (CIB1 Human, Mustard Weed)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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CuO-LacI-NLS–SNAPf

Plasmid

#227368

Purpose

Lentiviral expression of LacI–SNAP

Depositor

David Pellman

Article

Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

Insert

LacI NLS SNAPf

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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AZ64_pE.DonorCLYBL.TS

Plasmid

#199228

Purpose

Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

Expression unit for EGFP reporter

Use

Gene targeting donor plasmid

Expression

Mammalian

Promoter

Human PGK1 gene promoter

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-pLtetO-gfp

Plasmid

#89476

Purpose

Broad-host plasmid expressing gfp under pLtetO promoter

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pLtetO-GFPmut3b

Promoter

pLtetO

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pORF1

Plasmid

#206025

Purpose

An expression vector for mouse LINE1 ORF1

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

ORF1 derived from mouse LINE1

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPER TBK1

Plasmid

#26210

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER TBK1 (TBK1 Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-FlaviA-mNeptune-puro

Plasmid

#140091

Purpose

Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

FlaviA-mNeptune

Use

Lentiviral; High expression

Tags

mNeptune

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-6XHis-SUMO-SIRT2

Plasmid

#102622

Purpose

Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Tags

6XHis-SUMO

Expression

Bacterial

Mutation

Deleted amino acids 1-37 and 357-389

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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