We narrowed to 7,668 results for: CCH

1. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-eGFP-SynaptoZip

   Plasmid

   #122525

   Purpose

   AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

   Depositor

   Antonio Malgaroli

   Article

   Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

   Insert

   eGFP-SynaptoZip (Vamp2 Rat)
   

   Use

   AAV

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWzyE-distrepII

   Plasmid

   #196667

   Purpose

   Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Tags

   TEV-cleavable di-strep tag II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet-1 TIM8,13

   Plasmid

   #170282

   Purpose

   Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

   Insert

   yeast Tim8 and Tim13 (TIM8 Budding Yeast)
   

   Tags

   TEV-cleavable N-terminal His tag on Tim13 (on bac…

   Expression

   Bacterial

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET151-Vps4(101-437)-Hcp1

   Plasmid

   #87737

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST1374-D10A-GCN4

   Plasmid

   #113025

   Purpose

   Expresses D10A-GCN4 in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

   Insert

   D10A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
   

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMV

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179051

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-RPC19-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIp128-CUP1-NUbo-GFP-His6

   Plasmid

   #212790

   Purpose

   Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-GFP-His6
   

   Use

   Integrative vector

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEasyG1_nat

   Plasmid

   #184911

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIMVAd39T

    Plasmid

    #98303

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAEL007097-Cas9

    Plasmid

    #100705

    Purpose

    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL007097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGAN200

    Plasmid

    #129203

    Purpose

    yTRAP sensor of [PSI+], detects aggregation of Sup35

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35NM (SUP35 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    N terminal and middle domains

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFL-EGFP-3xFlag-Fml1

    Plasmid

    #193058

    Purpose

    Baculovirus expression of S.pombe Fml1 for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Fml1 (fml1 Fission Yeast)
    

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Tags

    mRuby3

    Expression

    Yeast

    Promoter

    pHis3

    Available Since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-hPGK-mCherry-SynaptoZip

    Plasmid

    #177317

    Purpose

    Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)

    Plasmid

    #179054

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-TUB1
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

    Plasmid

    #176400

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    FRB-RGG-GFP-RGG-RGG
    

    Tags

    FRB and GFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS313_ATP3

    Plasmid

    #120253

    Purpose

    Expression of ATP3 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ATP3

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAEL003877-Cas9

    Plasmid

    #100581

    Purpose

    The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL003877-hspcas9-GFP
    Opie-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL003877 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-MED8

    Plasmid

    #15406

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED8 (MED8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only