We narrowed to 15,723 results for: LEA

1. pEF Doc2beta 6X mutant GFP

   Plasmid

   #128823

   Purpose

   To visualize Doc2beta 6X mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D163A, D218A, D220N, D303A, D357A, D359A

   Promoter

   EF-1alpha

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMGF157

   Plasmid

   #96972

   Purpose

   ish1-mCherry insertion cassette with Hygro resistance gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + hygro…

   Promoter

   N/A

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. NG1301 pCI-TPI-4MS2-SMG5(short)-4H

   Plasmid

   #65806

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Tags

   4 MS2 stem loops, SMG5 3'UTR shortened versi…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1a-V5-Mfsd10

   Plasmid

   #120238

   Purpose

   Expresses V5-tagged Mfsd10 in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Mfsd10 (Mfsd10 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABEhygro-Flag-ZO-2-delta-N

   Plasmid

   #27476

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of coding nucleotides from 1-351. This r…

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

6. GFP-DroshaS300A

   Plasmid

   #62526

   Purpose

   Serine 300 of Drosha is mutated to alanine

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serine 300 changed to alanine (DROSHA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serine 300 to alanine

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

7. GFP-DroshaS302A

   Plasmid

   #62527

   Purpose

   Serine 302 of Drosha is mutated to alanine

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serine 302 changed to alanine (DROSHA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serine 302 to alanine

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

8. PD-1-miSFIT-1-A, 5-A

   Plasmid

   #124682

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCin4-3xFLAG-MKL1 L272A

   Plasmid

   #19850

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 L272A (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Leucine 272 to Alanine

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

10. NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75233

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCin4-3xFLAG-MKL1 D444-630

    Plasmid

    #19840

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 D444-630 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 444-630

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5 FRT TO myc Separase delta 55/S1126A

    Plasmid

    #59825

    Purpose

    Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/S1126A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. HOXB9 (murine) HIS-tag pET

    Plasmid

    #8541

    Depositor

    Corey Largman

    Article

    Chang et al Mol Cell Biol 1996 Apr;16(4):1734-45.

    Insert

    HOXB9 (Hoxb9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Missing the first 13 amino acids of HOXB9; makes …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pCin4-3xFLAG-MKL1 S449A

    Plasmid

    #19842

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449 to Alanine

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pBacPak-HA-Non-stop

    Plasmid

    #52289

    Purpose

    Expresses Drosophila Non-stop

    Depositor

    Jerry Workman

    Article

    Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

    Insert

    Non-stop (not Fly)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. sgGCAT_2

    Plasmid

    #72885

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGCAT
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pMGF170

    Plasmid

    #96997

    Purpose

    ish1-mCherry insertion cassette with NAT resistance gene

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + NAT r…

    Promoter

    N/A

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiex PKC - Peptide 14 mCit mCer

    Plasmid

    #83565

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PKC alpha (PRKCA Human)
    

    Tags

    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MDH1-miR-221SM-PGK-GFP-Blast

    Plasmid

    #31520

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-221 (Mir221 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

20. MDH1-miR-196a-1SM-PGK-GFP-Blast

    Plasmid

    #31510

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a1 (Mir196a-1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only