We narrowed to 11,809 results for: emb
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Plasmid#171013PurposeEctopic expression of nonfluorescent BFPDepositorInsertuntagged nfBFP
UseLentiviralExpressionMammalianMutationBFP[H66A]PromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK2067
Plasmid#171845PurposeC. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)DepositorInserthis-58, eGFP, mCherry
TagsN-terminal Tag OLLAS, V5 and C-terminal Tag PEST…ExpressionWormMutationboxB wild type and hairpin mutantPromotermex-5 promoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(K8,9A)-mEGFP
Plasmid#168490PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP
ExpressionMammalianAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(K8,9A)-mPAGFP
Plasmid#168493PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP
ExpressionMammalianAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN15-mVenus
Plasmid#168486PurposeMammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertthe N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)
Plasmid#160577Purpose35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB)DepositorInsertPhiC31 PB (attP:TMtb:P35S:attB)
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP1Δ
Plasmid#160993PurposeExpress mouse p150 protein with PIP1 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
TagsGFPExpressionMammalianMutationPIP1 (PCNA-interacting protein) motif deletionAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTV-Met-Eif4a1-tdiRFP670-PEST
Plasmid#122371PurposeTargeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2A-tdiRFP-PEST-2A with homology arms for Eif4a1
UseMouse TargetingAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R360
Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-3
Plasmid#126054PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_YPet-PCP (y-plasmid)
Plasmid#127637PurposeEncodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.DepositorInsertYPet
UseSynthetic BiologyTagsRNA binding peptide: PP7 coat protein (PCP)ExpressionBacterialPromoterpJ21306Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-1
Plasmid#126056PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgMAPKAP1_2
Plasmid#124911PurposeTargeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9DepositorInsertMAPKAP1 (MAPKAP1 Human)
UseCRISPR and LentiviralAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgMAPKAP1_3
Plasmid#124913PurposeTargeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9DepositorInsertMAPKAP1 (MAPKAP1 Human)
UseCRISPR and LentiviralAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-ATG13[3SA](426)
Plasmid#122076PurposeExpressing HA-ATG13[3SA]DepositorInsertATG13
Tags3HAExpressionYeastMutationSerine 646 to alanine; Serine 649 to alanine; Ser…Available sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37deltaSEP
Plasmid#113501PurposeBacterial expression of GST-tagged p37 lacking the SEP domainDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 140-206Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-FLAG PLU-HIS
Plasmid#113173PurposeBaculovirus expression of PNG and PLUDepositorInsertPNG, PLU
UseBaculovirusAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-J6LE60
Plasmid#103117PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertJ6LE60(Cas9 coding gene from Rhodovulum sp. PH10)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-C7G697
Plasmid#103091PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertC7G697(Cas9 coding gene from Roseburia intestinalis L1-82)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D4J3S7
Plasmid#103096PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD4J3S7(Cas9 coding gene from Coprococcus catus GD/7)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only