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PHMcc(delta16 PHM 356s)

Plasmid

#25455

Depositor

Betty Eipper

Article

Kolhekar et al Biochemistry. 1997 Sep 9. 36(36):10901-9

Insert

PHM (Pam Rat)

Use

Tags

Expression

Mammalian

Mutation

Contains the catalytic core of PHM, including aa …

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
MERS-CoV spike first3KO mutant

Plasmid

#231922

Purpose

For expression of MERS-CoV spike protein with glycan deletion at N1176, N1213, N1225 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike first3KO (S MERS-CoV)

Use

Tags

Expression

Mammalian

Mutation

N1176Q, N1213Q, N1225Q

Promoter

CMV

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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MERS-CoV spike last4KO mutant

Plasmid

#231923

Purpose

For expression of MERS-CoV spike protein with glycan deletion at N1241, N1256, N1277, N1288 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike last4KO (S MERS-CoV)

Use

Tags

Expression

Mammalian

Mutation

N1241Q, N1256Q, N1277Q, N1288Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pJL1-AcrA

Plasmid

#198214

Purpose

pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

AcrA

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-PAH1-3HA

Plasmid

#184761

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

PAH1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-CDS1-3HA

Plasmid

#184760

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

CDS1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-DGK1-3HA

Plasmid

#184759

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

DGK1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PC(1-86)-H6

Plasmid

#183780

Purpose

Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Use

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHP003Env

Plasmid

#133985

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHP015Env

Plasmid

#133987

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHP051Env

Plasmid

#133995

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHP025Env

Plasmid

#133990

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHP038Env

Plasmid

#133992

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHP043Env

Plasmid

#133994

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHP024Env

Plasmid

#133989

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHP022Env

Plasmid

#133988

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHP013Env

Plasmid

#133986

Purpose

Env gene from HIV patient plasma in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-Hylight

Plasmid

#193447

Purpose

A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

Depositor

Richard Goodman

Article

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Hylight Biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO 3xFlag-m-PKD1-2xMyc/His

Plasmid

#83452

Purpose

Full-length murine PC1 for Eukaryotic Expression (982)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

polycystin-1 (Pkd1 Mouse)

Use

Tags

2xMyc, 3xFlag, and 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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