Addgene

We narrowed to 4,183 results for: 256

Showing: 1281 - 1320 of 4183 results

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FL-fibulin-3 pcDNA4

Plasmid

#29700

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
HS-Utrophin-GFP

Plasmid

#105952

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Utrophin (Utrn Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Cyto-RA

Plasmid

#209860

Purpose

Dimerization dependent fluorescent protein RA fused to NES

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Use

Tags

NES sequence NLVDLQKKLEELELDEQQ

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBG-TFII-I deltaBR

Plasmid

#22185

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

Insert

TFII-I (GTF2I Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Deleted amino acids 301-306

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
3xFNLDD/pMXs-IG

Plasmid

#48972

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

Insert

3xFNLDD

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Keratin 8 S73A

Plasmid

#18056

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

S73A

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I-delta N90

Plasmid

#22165

Depositor

Ananda Roy

Article

Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

Insert

TFII-I (GTF2I Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

The first 90 aa of TFII-I are deleted

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I beta

Plasmid

#22155

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
HS-DN-Plk1

Plasmid

#105949

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Plk1 (PLK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Dominant negative K82 R

Promoter

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Keratin 8 S73D

Plasmid

#18054

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

S73D

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pSH1/Sn-E-Fv'Fvls-E

Plasmid

#15274

Depositor

David Spencer

Article

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36) (FKBP1A Human)

Use

Tags

HA epitope

Expression

Mammalian

Mutation

FKBP12 F36V

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-II-I-EGFP delta isoform

Plasmid

#22149

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
mCitrine-VE-Cadherin-N-10

Plasmid

#56319

Purpose

Localization: Tight Junctions, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

VE-Cadherin (CDH5 Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

I503T in VE-Cadherin

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBXPHM3-PCFT_NB

Plasmid

#165415

Purpose

Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT_NB (SLC46A1 Llama, Chicken)

Use

Tags

His10-MBP-3C protease site

Expression

Bacterial

Mutation

Promoter

arabinose

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
7iBi89 (rat fibronectin)

Plasmid

#14065

Depositor

Richard Hynes

Article

Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

Insert

Rat Fibronectin 7iBi89 (Fn1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Insert was cut out of pGEM3 with SalI and SacI an…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
Keratin 8 S431A

Plasmid

#18058

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

S431A

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
3xFNLDD/pCMV-7.1

Plasmid

#48874

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

Insert

3xFNLDD

Use

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330(puro) Rosa26-H3F3B

Plasmid

#73131

Purpose

Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

spCas9

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-GFPγ-SpHis5

Plasmid

#60781

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPγ

Use

Yeast integrating

Tags

Expression

Mutation

T65C S72A V163A I167T L231H

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
human K8

Plasmid

#14742

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I N90

Plasmid

#22186

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

Insert

TFII-I (GTF2I Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Contains the first 90 aa of TFII-I

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pUHD Cdk2 WT HA

Plasmid

#27650

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Use

Tags

HA (YDVPDYA)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3_AKAR5Δ

Plasmid

#119912

Purpose

Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

AKAR5Δ

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5_tAKARγ

Plasmid

#119915

Purpose

Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARγ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p3.2mar-GFP

Plasmid

#63694

Purpose

3.2 kb marine armor plate enhancer driving GFP reporter

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

hsp70

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124287

Purpose

sgRNA against human Col7A1

Depositor

Csaba Vizler, Katalin Josvay

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Use

Tags

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Synthetic, Chicken)

Use

Tags

GFP-His8

Expression

Yeast

Mutation

Promoter

GAL1

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-II-I-deltaNLS1-EGFP

Plasmid

#22150

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 297-304

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEIIIA

Plasmid

#14059

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIA (Fn1 Rat)

Use

Tags

Expression

Mammalian

Mutation

EcoRI linkers added to TaqI to HinfI fragment

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pUHD Cdk2 DN HA

Plasmid

#27651

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Use

Tags

HA (YDVPDYA)

Expression

Mammalian

Mutation

D146N (Dominant Negative)

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pUAP1_Ta_Yr7_CDS_no_STOP

Plasmid

#141093

Purpose

Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

Depositor

Cristobal Uauy

Article

Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

Insert

Ta_Yr7

Use

Unspecified; Level 0 module for golden gate cloni…

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4pBS4-Pleum

Plasmid

#60329

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Keratin 8 G61C

Plasmid

#18055

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

G61C

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Keratin 8 S23A

Plasmid

#18057

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Use

Tags

Expression

Mammalian

Mutation

S23A

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pcFN-C (chick fibronectin)

Plasmid

#14063

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

DraI - EcoRI fragment inserted in HincII-EcoRI si…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS cCollagen XII (CT#1051)

Plasmid

#13916

Depositor

Cliff Tabin

Article

Brent et al Development. 2005 Feb . 132(3):515-28.

Insert

Collagen XII (COL12A1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2-C11orf83-V5

Plasmid

#65843

Purpose

Mammalian expression of C terminally V5-tagged C11orf83/UQCC3

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 WT (UQCC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

WT

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5_tAKARζ

Plasmid

#119918

Purpose

Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARζ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124286

Purpose

sgRNA against human Col7A1

Depositor

Csaba Vizler, Katalin Josvay

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1281 - 1320 of 4183 results

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