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We narrowed to 171,195 results for: addgene

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Showing: 1281 - 1320 of 171195 results

  1. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  2. 6ASB (FBXL19)

    Plasmid
    #119954
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    FBXL19 (FBXL19 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 31-153
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119954-plasmid-map-sequence-id-232625

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  5. USP21 (3MTN)

    Plasmid
    #28112
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP21 (USP21 Human)
    Tags
    His tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    catalytic domain only (amino acid residues 209-56…
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pELK3.1.0-gDNA

    Plasmid
    #112418
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3
    Depositor
    Kevin White
    Insert
    ELK3 (ELK3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112418-plasmid-map-sequence-id-230297

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  7. KCNK2

    Plasmid
    #73257
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KCNK2 (KCNK2 Human)
    Tags
    TEV-His10-FLAG
    Expression
    Insect
    Mutation
    userdefined:Full_Length 1411
    Promoter
    Polyhedrin
    Available Since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    73257-plasmid-map-sequence-id-150197

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  8. CBX2

    Plasmid
    #25158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX2_04 (CBX2 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25158-plasmid-map-sequence-id-472908

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  9. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  10. FGD5

    Plasmid
    #28109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FGD5:HPC09X-H08:C212499 (FGD5 Human)
    Tags
    His-tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 889-1304.
    Available Since
    March 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. CDK12

    Plasmid
    #73258
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CDK12 (CDK12 Human)
    Tags
    His-6-TEV
    Expression
    Insect
    Mutation
    pfam00069: Pkinase 727-1020
    Promoter
    Polyhedrin
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. GALTA-c001

    Plasmid
    #79645
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GALT (GALT Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79645-plasmid-map-sequence-id-150209

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  13. pBabe H-Ras V12 C40

    Plasmid
    #18747
    Depositor
    Scott Lowe
    Insert
    H-Ras V12 C40 (HRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    V12 C40
    Available Since
    July 7, 2008
    Availability
    Academic Institutions and Nonprofits only

  14. p-dCas9-DNMT3a-Hygro

    Plasmid
    #104403
    Purpose
    transient expression of dCas9-DNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    DNMT3a (DNMT3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104403-plasmid-map-sequence-id-204468

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  15. FEM1C

    Plasmid
    #167842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167842-plasmid-map-sequence-id-331056

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  16. pBabe H-Ras V12 S35

    Plasmid
    #18746
    Depositor
    Scott Lowe
    Insert
    H-Ras V12 S35 (HRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    V12 S35
    Available Since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only

  18. SMARCA2

    Plasmid
    #73250
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SMARCA2B (SMARCA2 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73250-plasmid-map-sequence-id-136215

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  19. pBabe H-Ras V12 G37

    Plasmid
    #18745
    Depositor
    Scott Lowe
    Insert
    H-Ras V12 C40 (HRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    V12 / G37
    Available Since
    July 7, 2008
    Availability
    Academic Institutions and Nonprofits only

  20. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Available Since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

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  21. RBBP4

    Plasmid
    #25350
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RBBP4-13 (RBBP4 Human)
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pIVT-myc-T58A-S62D

    Plasmid
    #138540
    Purpose
    For making myc-T58A-S62D RNA
    Depositor
    Carmen Williams
    Insert
    Myelocytomatosis oncogene T58A S62D (Myc Mouse)
    Use
    In vitro transcription
    Mutation
    Threonine 58 changed to alanine and serine 62 cha…
    Promoter
    T7
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    138540-plasmid-map-sequence-id-291746

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  23. 4RFQ

    Plasmid
    #64656
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL18:PBC004-E03:C231318 (METTL18 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    May 15, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pERF.1.0-gDNA

    Plasmid
    #112395
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERF
    Depositor
    Kevin White
    Insert
    ERF (ERF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112395-plasmid-map-sequence-id-230242

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  27. pBCL6.1.0-gDNA

    Plasmid
    #112485
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6
    Depositor
    Kevin White
    Insert
    BCL6 (BCL6 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112485-plasmid-map-sequence-id-230527

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  28. MCTS1

    Plasmid
    #32049
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MCTS1 (MCTS1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    E137A, K139A, Q140A
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. 6OGK

    Plasmid
    #162259
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    MECP2 (MECP2 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162259-plasmid-map-sequence-id-319832

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  30. pE2F3.3.1-gDNA

    Plasmid
    #113776
    Purpose
    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3
    Depositor
    Kevin White
    Insert
    E2F3 (E2F3 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113776-plasmid-map-sequence-id-230565

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  31. 5T1I (CBX3)

    Plasmid
    #101885
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX3 (CBX3 Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101885-plasmid-map-sequence-id-194721

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  32. 4YZ8

    Plasmid
    #69955
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WHSC1L1:JMC055-F10:C223308 (NSD3 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. 5THA (Gemin5)

    Plasmid
    #101890
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Gemin5 (GEMIN5 Human)
    Use
    Baculovirus expression
    Tags
    6xHis, Thrombin cleavage
    Promoter
    polyhedrin
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101890-plasmid-map-sequence-id-194723

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  34. 5W9S (CXXC5)

    Plasmid
    #101266
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC5 (CXXC5 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    101266-plasmid-map-sequence-id-193499

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  35. KNS2

    Plasmid
    #28122
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KNS2:HPC09H-C05:C207000 (KLC1 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Available Since
    March 22, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. pKG204

    Plasmid
    #63078
    Purpose
    Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    HP1 alpha (CBX5 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    V151I mutation compared to GenBank reference sequ…
    Available Since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63078-plasmid-map-sequence-id-134726

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  37. PHF20 (3QII)

    Plasmid
    #32039
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF20 (PHF20 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Tudor domain 2 only (amino acids 83-150)
    Promoter
    T7
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. HLTF

    Plasmid
    #28152
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    helicase-like transcription factor (HLTF Human)
    Tags
    thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains HIRAN domain (residues 51 to 171).
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. 5C7M (ITCH)

    Plasmid
    #69966
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH:SDC094-D06:C29524 (ITCH Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. DDI1

    Plasmid
    #32867
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DDI1 (DDI1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    amino acids 239-367 only
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1281 - 1320 of 171195 results