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Showing: 1601 - 1650 of 8645 results

  6. pGL4.10/RSV_XS3B-miniXon_Luciferase

    Plasmid
    #217302
    Purpose
    Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by L
    Depositor
    Beverly L. Davidson
    Insert
    XS3B-miniXon
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    217302-plasmid-map-sequence-id-436485

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  14. S111

    Plasmid
    #174549
    Purpose
    Constitutive PhCMV-driven mammalian expression vector of CDH-1-TF cassette (PhCMV-Gal4-Bcl-XL-P2A-LD3-Rel65-pA)
    Depositor
    Bruno Correia
    Article
    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:
    Insert
    BclxL and designed protein LD3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174549-plasmid-map-sequence-id-343591

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  15. S112

    Plasmid
    #174550
    Purpose
    Constitutive PhCMV-driven mammalian expression vector of CDH-2-TF cassette (PhCMV-Gal4-Bcl2-P2A-LD3-Rel65-pA)
    Depositor
    Bruno Correia
    Article
    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:
    Insert
    Bcl2 and designed protein LD3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174550-plasmid-map-sequence-id-343592

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  17. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  21. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  23. pCIFR-3

    Plasmid
    #233295
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Promoter
    p14G
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233295-plasmid-map-sequence-id-468475

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  26. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

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  29. pGS1222

    Plasmid
    #63791
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eGFP-Twin-Strep tag
    Promoter
    none
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63791-plasmid-map-sequence-id-106478

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  33. pGS1011

    Plasmid
    #63783
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Strep-tag II followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Strep-tag II
    Promoter
    none
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63783-plasmid-map-sequence-id-106469

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  34. pCIFR-8

    Plasmid
    #227666
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    AmR
    Expression
    Bacterial
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227666-plasmid-map-sequence-id-458899

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  37. pGS1623

    Plasmid
    #63795
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by HIS5 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    tDimer2-Twin-Strep tag followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Twin-Strep
    Promoter
    none
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63795-plasmid-map-sequence-id-110439

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  38. pGS1322

    Plasmid
    #63792
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCFP-Twin-tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eCFP-Twin-tag
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63792-plasmid-map-sequence-id-106481

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  39. pGS1112

    Plasmid
    #63784
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    Sapphire-Strep-tag II
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63784-plasmid-map-sequence-id-106474

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  40. pGS1522

    Plasmid
    #63794
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eVenus-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eVenus-Twin-Strep
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63794-plasmid-map-sequence-id-106482

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  41. pGS1422

    Plasmid
    #63793
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Twin-tag followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Twin-tag
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63793-plasmid-map-sequence-id-106479

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  42. pGS1512

    Plasmid
    #63788
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eVenus-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    eVenus-Strep-tag II
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63788-plasmid-map-sequence-id-106476

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  43. pGS1312

    Plasmid
    #63786
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCFP-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    eCFP-Strep-tag II
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63786-plasmid-map-sequence-id-106475

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  46. pYES2-gRNA-hyg-MCS

    Plasmid
    #107734
    Purpose
    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.
    Depositor
    Sheng Yang
    Article
    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.
    Insert
    X-3 site gRNA expression cassettes
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    SNR52p
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    107734-plasmid-map-sequence-id-208539

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  50. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid
    #91249
    Purpose
    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    91249-plasmid-map-sequence-id-181276

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Showing: 1601 - 1650 of 8645 results