We narrowed to 1,621 results for: nif

1. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

   Plasmid

   #236239

   Purpose

   Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   CaV2.1 (CACNA1A Rabbit)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV hSyn INTRON mEmerald-ER WPRE

   Plasmid

   #236230

   Purpose

   AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
   

   Use

   AAV

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV hSyn INTRON HaloTag-Sec61b WPRE

   Plasmid

   #236229

   Purpose

   AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   HaloTag-Sec61b (SEC61B Synthetic, Human)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

   Plasmid

   #236245

   Purpose

   Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   U6 and human Synapsin1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

   Plasmid

   #236240

   Purpose

   Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   CaV2.2 (CACNA1B Rabbit)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV hSyn INTRON mEmerald-Sec61b WPRE

   Plasmid

   #236228

   Purpose

   AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mEmerald-Sec61b (SEC61B Synthetic, Human)
   

   Use

   AAV

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

   Plasmid

   #163701

   Purpose

   Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Inserts

   SOX2-3xHA-P2A-tagBFP (SOX2 Human)
   rtTA
   T2-sgRNA
   

   Use

   AAV and CRISPR

   Tags

   3x-HA and P2A-tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE3G

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-CRY2oligo-mcherry-ANXA11

   Plasmid

   #164203

   Purpose

   Expresses CRY2oligo-mcherry tagged ANXA11 under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Tags

   CRY2oligo-mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-CRY2oligo-mcherry-ANXA11(R235Q)

   Plasmid

   #164205

   Purpose

   Expresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Tags

   CRY2oligo-mcherry

   Expression

   Mammalian

   Mutation

   R235Q

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET_His6-SUMO-TEV-TtCsm6

    Plasmid

    #172486

    Purpose

    Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
    

    Use

    Tags

    His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-CRY2oligo-mcherry-ANXA11(D40G)

    Plasmid

    #164204

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    D40G

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBFC1207

    Plasmid

    #186694

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIRES2-smGFP-Kv2.1

    Plasmid

    #131709

    Purpose

    Expresses Kv2.1 in mammalian cells

    Depositor

    Jennifer Kearney

    Article

    Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

    Insert

    KCNB1 (KCNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX-EF1a ANXA11-mEmerald D40G

    Plasmid

    #164211

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    D40G

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)

    Plasmid

    #164208

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    186-505 aa

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-CRY2oligo-mcherry-ANXA11(1-185aa)

    Plasmid

    #164207

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    1-185 aa

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-CRY2oligo-mcherry-ANXA11(R346C)

    Plasmid

    #164206

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 R346C mutant under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    R346C

    Promoter

    CMV

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLEX-EF1a ANXA11-mEmerald R346C

    Plasmid

    #164213

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R346C

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLEX-EF1a ANXA11-mEmerald R235Q

    Plasmid

    #164212

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R235Q

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV hSyn INTRON mScarlet-STIM2 WPRE

    Plasmid

    #236236

    Purpose

    AAV expression of human STIM2 internally tagged with mScarlet-I

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mScarletI-STIM2 (STIM2 Human)
    

    Use

    AAV

    Tags

    mScarletI in position 29

    Expression

    Mutation

    Signal peptide from STIM1 and residues 15-746 of …

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV hSyn INTRON HaloTag-STIM2 WPRE

    Plasmid

    #236235

    Purpose

    AAV expression of human STIM2 internally tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    HaloTag-STIM2 (STIM2 Human)
    

    Use

    AAV

    Tags

    HaloTag in position 29

    Expression

    Mutation

    Signal peptide from STIM1 and residues 15-746 of …

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge