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pTM1_VP35_ZEBOV

Plasmid

#68121

Purpose

Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

VP35 ZEBOV (VP35 )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pKNOCK-Tc

Plasmid

#46259

Purpose

Bacterial targeting

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

Type

Empty backbone

Use

Bacterial targeting

Available Since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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GUM BUB3

Plasmid

#84029

Purpose

Expression of mCherry tagged BUB3 in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BUB3 (BUB3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

UCOE / SFFV

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pOSIP-CT (CamR, P21)

Plasmid

#45981

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pQUAST-mtdT-3XHA

Plasmid

#24354

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

mtDT-3XHA

Expression

Insect

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pQUAS-GG

Plasmid

#24336

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

5xQUAS/GFP split with an intron (GG)

Expression

Mammalian

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP

Plasmid

#64111

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ134B-TLS

Plasmid

#245879

Purpose

Golden gate entry vector carrying TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

TLS2

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ132B-PtALSgRNA

Plasmid

#245880

Purpose

Golden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

PtALSgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ133B-TLS

Plasmid

#245882

Purpose

Golden gate entry vector carrying TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

TLS2

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ131-FCY-UPP

Plasmid

#245873

Purpose

Golden gate entry vector carrying expression cassette for genes FCY and UPP

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

FCY1-UPP

Use

CRISPR

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ132B-CsALSgR1.1

Plasmid

#245875

Purpose

Golden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

CsALS_gRNA1.1

Use

CRISPR; Golden gate entry vector to expressing t…

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ133B-CsALSgR1.2

Plasmid

#245876

Purpose

Golden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS gene

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

CsALSgR1.2

Use

CRISPR; Golden gate entry vector

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pMT-FLAG-CBPΔN

Plasmid

#182840

Purpose

A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

Depositor

Peter Harte

Article

Tie et al Development. 2009 Sep;136(18):3131-41. d

Insert

CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)

Tags

FLAG tag

Expression

Insect

Promoter

MT

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTM1_VP30_ZEBOV

Plasmid

#69119

Purpose

Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

VP30 ZEBOV (VP30 )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pRB17

Plasmid

#52527

Purpose

serves as template for PCR-mediated fusion of U6-promoter with sgRNA

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Drosophila U6C promoter

Use

CRISPR; Blunt cloning kit

Tags

T7-promoter

Expression

Insect

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCFJ355 - MCS(ttTi14024, X)

Plasmid

#34870

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi14024 targeting

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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