We narrowed to 11,191 results for: Vars;

1. pBiex-1 AC30_eGFP

   Plasmid

   #82715

   Purpose

   Expresses a GFP-tagged recombinant adenylyl cyclase.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4497

   Plasmid

   #80339

   Purpose

   mammalian expression FnCpf1 nuclease and Fn crRNA

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   FnCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE4889

   Plasmid

   #88905

   Purpose

   Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(D917A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE4566

   Plasmid

   #88904

   Purpose

   Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   hMbCpf1(D986A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSPL3-hRPH3A_c.444Gwt

   Plasmid

   #190476

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSPL3-hRPH3A_c.1853Gmut

   Plasmid

   #190479

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.1853A>G

   Promoter

   SV40

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBiex1-SYNAC

   Plasmid

   #82710

   Purpose

   Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

   Insert

   SYNAC (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE3328

   Plasmid

   #107539

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K607R

   Promoter

   CMV and human U6

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE3329

   Plasmid

   #107538

   Purpose

   Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Lb crRNA
   hLbCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K595R

   Promoter

   CMV and human U6

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE3327

    Plasmid

    #107535

    Purpose

    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    hAsCpf1(RVR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    S542R, K548V, N552R

    Promoter

    CMV and human U6

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTE3334

    Plasmid

    #107537

    Purpose

    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K582V, N586R

    Promoter

    CMV and human U6

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE3330

    Plasmid

    #107534

    Purpose

    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    hLbCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV and human U6

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTE3336

    Plasmid

    #107541

    Purpose

    Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(RR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K637R

    Promoter

    CMV and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTE3333

    Plasmid

    #107540

    Purpose

    Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    hFnCpf1(RR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K671R

    Promoter

    CMV and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTE3331

    Plasmid

    #107536

    Purpose

    Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    hFnCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K613V, N617R

    Promoter

    CMV and human U6

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSPL3-hRPH3A_c.444Tmut

    Plasmid

    #190477

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.444G>T

    Promoter

    SV40

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSPL3-hRPH3A_c.1853Awt

    Plasmid

    #190478

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX458-3xHA-SpCas9-HF1

    Plasmid

    #201952

    Purpose

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pαH-S-GSAS-D614G-deltaFV

    Plasmid

    #171743

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MKC89_CCR5(UbC-tEPOR-2A-YFP)

    Plasmid

    #232404

    Purpose

    AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.

    Depositor

    Matthew Porteus, Kyle Cromer

    Article

    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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