We narrowed to 1,085 results for: cas12a

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pGBScomp Low

Plasmid

#223203

Purpose

Group B Streptococcus Low Expression Modular Complementation Vector

Depositor

Thomas Hooven

Article

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

Complementation vector

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ11350

Plasmid

#183207

Purpose

PB-TRE3G-BFP-Triplex-crGFP*ABE3-SV40pA

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYZ673

Plasmid

#132953

Purpose

crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

Insert

FnCas12a

Use

CRISPR and Synthetic Biology

Promoter

adh1

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217345

Purpose

crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ11429

Plasmid

#183205

Purpose

pHR-R(NFKB-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJYScr

Plasmid

#132719

Purpose

Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

Depositor

Jan Marienhagen

Article

Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z31

Plasmid

#120074

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z12

Plasmid

#120055

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z09

Plasmid

#120052

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P27

Plasmid

#120038

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P24

Plasmid

#120035

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P17

Plasmid

#120028

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P08

Plasmid

#120019

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-15

Plasmid

#119994

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

Plasmid

#115220

Purpose

Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin3Cas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

Plasmid

#115207

Purpose

Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

BzoCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ11444

Plasmid

#183628

Purpose

pHR-R(AP1-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-11

Plasmid

#119990

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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