-
Plasmid#83523PurposeAromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 geneDepositorInsertpARO9-mfalpha1
UseTagsExpressionYeastMutationPromoterpARO9Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW005
Plasmid#83524Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 geneDepositorInsertpFUS1-malpha1
UseTagsExpressionYeastMutationPromoterpFUS1Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MLM3720
Plasmid#49944PurposeExpresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a controlDepositorInsert3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
UseTALENTags3x FLAGExpressionMutationcatalytic domain of TET1 inactive (H1671Y and D16ā¦PromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTK194
Plasmid#59563PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak mKate opal attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK313
Plasmid#59570PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK317
Plasmid#59571PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK312
Plasmid#59569PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak EGFP ochre attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-PIK3CD
Plasmid#203730PurposeAAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoterDepositorInsertPIK3CD (PIK3CD Human)
UseAAVTagsExpressionMammalianMutationPromoterHuman synapsin promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-gSM
Plasmid#215870PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInsert3 tandem repeats of target site (complementary to seed seq of guide strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRKCDPB_pLX307
Plasmid#98359PurposeLentiviral expression of PRKCDPBDepositorInsertPRKCDPB (CAVIN3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationR8PPromoterE1FaAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pSM
Plasmid#215871PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInsert3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET42a-lambdaN+-L+-GSH
Plasmid#98894PurposeExpresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo methodDepositorInsertlambdaN+-L+
UseTagsGST tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-CDC25A
Plasmid#216511PurposeExpresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoterDepositorInsertCDC25A (CDC25A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KAC1001(D1957A)
Plasmid#139312PurposeRecruitable PI4KA kinase-dead catalytic fragmentDepositorInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
UseTagsExpressionMammalianMutationD1957A, G1986DPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KAC1001
Plasmid#139311PurposeRecruitable PI4KA catalytic fragmentDepositorInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
UseTagsExpressionMammalianMutationG1986DPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Clomeleon
Plasmid#203723PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentrationDepositorInsertcyan fluorescent protein linked yellow florescent protein
UseTagsExpressionBacterial and MammalianMutationCyan Fluorescent Protein, Topaz Fluorescent Proteā¦PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G0-R20
Plasmid#101818PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G0-R11
Plasmid#101808PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G0-R18
Plasmid#101813PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-VC3AI
Plasmid#78907PurposeIndicator of caspase-3-like protease activityDepositorInsertVC3AI
UseLentiviralTagsHA and MycExpressionMutationS320A, deletion of AA1-200PromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only