We narrowed to 1,242 results for: membrane gfp

1. pPyPGK-EGFP-TM-IRES-Pac

   Plasmid

   #183604

   Purpose

   Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Use

   Tags

   Human PDGFRB transmembrane domain and Mouse IgGK …

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Pgk1

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus-7aa-ActA-pEGFP-C1

   Plasmid

   #177405

   Purpose

   To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

   Depositor

   David Andrews

   Article

   Osterlund et al Protocol Exchange (2022)

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX

   Plasmid

   #202718

   Purpose

   Mammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Pink Flamindo
   paGFP-bPAC-CAAX
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

   Plasmid

   #202717

   Purpose

   Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Myr-Pink Flamindo
   EGFP-bPAC
   

   Use

   Lentiviral

   Tags

   10xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LifeAct-GFP-Fis1

   Plasmid

   #182576

   Purpose

   Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-Fis1
   

   Use

   Tags

   Fis1 and LifeAct

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIBN(deltaNLS)-pmGFP

   Plasmid

   #26867

   Purpose

   Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   NLS sequences mutated

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenSyn2-EGFP-­OMP25C

   Plasmid

   #71427

   Purpose

   Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

   Depositor

   Henri Huttunen

   Article

   Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

   Insert

   OMP25 (172-­‐206) (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 172-206 only

   Promoter

   synapsin

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Arch(D95H)-eGFP

   Plasmid

   #51081

   Purpose

   Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

   Depositor

   Adam Cohen

   Article

   Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:

   Insert

   Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Aspartic Acid 95 to Histidine

   Promoter

   Ubiquitin

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-acVHH (Y34W)-PB

   Plasmid

   #171072

   Purpose

   A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

   Depositor

   Yubin Zhou

   Article

   Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

   Insert

   anti-caffeine nanobody variant
   

   Use

   Tags

   modified STIM1–PB tag

   Expression

   Mammalian

   Mutation

   Y34W

   Promoter

   CMV

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-acVHH (Y34F)-PB

    Plasmid

    #171071

    Purpose

    A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

    Depositor

    Yubin Zhou

    Article

    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

    Insert

    anti-caffeine nanobody variant
    

    Use

    Tags

    modified STIM1–PB tag

    Expression

    Mammalian

    Mutation

    Y34F

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP–acVHH (Y104F)–PB

    Plasmid

    #169683

    Purpose

    A COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

    Depositor

    Yubin Zhou

    Article

    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

    Insert

    anti-caffeine nanobody variant
    

    Use

    Tags

    modified STIM1–PB tag

    Expression

    Mammalian

    Mutation

    Y104F

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LVDP 2P.2.EGFP

    Plasmid

    #231905

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPs

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, E, M SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R203M in N protein

    Promoter

    CMV, EF-1a

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

    Plasmid

    #213402

    Purpose

    douple floxed, myristoylation (myr) site for membrane targeting and EGFP

    Depositor

    Katrin Willig

    Article

    Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

    Insert

    EGFP
    

    Use

    AAV

    Tags

    C-terminal (Ct) cytoplasmic domains of low densit…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    UAS

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183605

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Use

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bet1-GFP

    Plasmid

    #209850

    Purpose

    Expresses Bet1-GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Bet1 (BET1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tet-myr-CSK-GFP

    Plasmid

    #83470

    Purpose

    Lentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    CSK (CSK Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline-inducible promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMP71-mGFP-LLO-mPMEL

    Plasmid

    #174608

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    membrane GFP fused to LLO and mouse PMEL antigen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.0_LynLAMA-G97 IRES EGFP

    Plasmid

    #130711

    Purpose

    Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP
    

    Use

    Tags

    Lyn11 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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