We narrowed to 5,654 results for: pCas

1. pCAG-C-CreintG

   Plasmid

   #69573

   Purpose

   One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

   Insert

   kozak consensus - C-CreintG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mRuby2

   Plasmid

   #89686

   Purpose

   pCAG promoter driving mRuby2

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-RNF169

   Plasmid

   #119004

   Purpose

   Mammalian expression of human RNF169 and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   RNF169 (RNF169 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-ATG14

   Plasmid

   #203565

   Purpose

   Mammalian expression of ATG14

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG14 (ATG14 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAD39_mIRG1_4-462_pvp008

   Plasmid

   #124873

   Purpose

   Expresses mouse CAD (Irg1) in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (Acod1 Mouse)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-3 and 463-end.

   Promoter

   T7

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG--uMASS_LF

   Plasmid

   #209763

   Purpose

   Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   uMASS_LF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-uMASS_1

   Plasmid

   #209762

   Purpose

   Expression of uMASS_1 in mammalian cells under CAG promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   uMASS_1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-p21_WT

   Plasmid

   #40202

   Depositor

   Philipp Mergenthaler

   Article

   Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CAG

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMA-SpCas9-g7

   Plasmid

   #80790

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Gdf5

    Plasmid

    #163596

    Purpose

    Expresses GDF-5 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf5 (Gdf5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9 TadCBEc

    Plasmid

    #193834

    Purpose

    Express TadCBEc (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDc-SpCas9 D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Chimeric_Butterfly_CY_1.0

    Plasmid

    #59800

    Purpose

    Genetically encoded fluorescent voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

    Insert

    Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Bmp8a

    Plasmid

    #163592

    Purpose

    Expresses BMP-8A in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp8a (Bmp8a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL2621 (pCas8_Vch)

    Plasmid

    #200893

    Purpose

    Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas8
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL2623 (pCas6_Vch)

    Plasmid

    #200895

    Purpose

    Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas6
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Bmp7

    Plasmid

    #163591

    Purpose

    Expresses BMP-7 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp7 (Bmp7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-HASAP1-ST

    Plasmid

    #138965

    Purpose

    chemigenetic voltage indicator, soma targeted

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Insert

    HASAP1-ST
    

    Use

    Tags

    somatic localization tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mutation

    Promoter

    TgTUB1

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSpCas9-EF-5Pou5f1

    Plasmid

    #174872

    Purpose

    CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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