We narrowed to 6,045 results for: pCas

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pCAFNF-DsRed

Plasmid

#13771

Purpose

Flp recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

DsRed2

Use

Flp/frt

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-CyRFP1

Plasmid

#84356

Purpose

Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

CyRFP1

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGIG-CDKN1A

Plasmid

#228912

Purpose

Expression CDKN1A in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

Cdkn1a (Cdkn1a Mouse)

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10B-HA

Plasmid

#209216

Purpose

Mammalian expression of ATP10B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP10B (ATP10B Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDNR-SpCas9

Plasmid

#80425

Purpose

Used for in vitro transcription of SpCas9 mRNA.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9

Use

CRISPR

Tags

none

Mutation

codon optimized for human

Promoter

T7

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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AV15_pCAG.Cas9.gRNA.S1

Plasmid

#199259

Purpose

Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGG-NFIX2

Plasmid

#112699

Purpose

Expression of NFIX2 protein and GFP

Depositor

Richard Gronostajski, Francois Guillemot

Article

Martynoga et al Genes Dev. 2013 Aug 15;27(16):1769-86. d

Insert

Nuclear Factor I X2 (Nfix Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-NCad-V

Plasmid

#191665

Purpose

mVenus-inserted mouse N-cadherin

Depositor

Takeharu Nagai

Article

Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

Insert

N-cadherin

Tags

HA and mVenus

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1A

Plasmid

#15227

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert A (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-TAG

Plasmid

#26771

Depositor

Shankar Srinivas

Article

Trichas et al BMC Biol. 2008 Sep 15;6:40. doi: 10.1186

Insert

CAG-TdTomato-2A-H2BGFP-SV40pA

Expression

Bacterial

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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LLP777_SnoopCatcher-p65HSF1-CO

Plasmid

#211770

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SnoopCatcher-p65HSF1

Tags

2xOLLAS

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1a-nSpCas9

Plasmid

#171691

Purpose

Expresses nSpCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

nSpCas9

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

EF1a

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-hCas9D10A

Plasmid

#52101

Purpose

The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter

Depositor

Izuho Hatada

Insert

hCas9D10A

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available Since

April 30, 2014

Availability

Academic Institutions and Nonprofits only
pCAGGs-Foxp1

Plasmid

#45720

Depositor

Jeremy Dasen

Article

Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

Insert

FoxP1 (Foxp1 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-Kv-ArcLight-ST

Plasmid

#182319

Purpose

Soma-targeted fluorescent voltage indicator with left-shifted voltage-dependency

Depositor

Rafael Yuste

Article

Bando et al Nat Commun. 2021 Dec 10;12(1):7229. doi:

Insert

Kv-ArcLight-ST

Expression

Mammalian

Mutation

Mutation in an amino acid Q217E of ArcLight-MT

Promoter

CAG

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS_HA-VP35_EBOV

Plasmid

#103053

Purpose

Mammalian expression vector encoding HA-tagged Ebola virus VP35 gene under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al J Virol. 2016 Jul 27;90(16):7268-84. doi

Insert

VP35 (VP35 Zaire ebolavirus, Mayinga)

Tags

HA-tag

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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B-SpCas9-HF1

Plasmid

#126762

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-SpCas9-HF1

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Mammalian_SpCas9_Target-AID2S

Plasmid

#188648

Purpose

Expresses SpCas9_Target-AID2S in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SpCas9

Promoter

pCMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FALSE

Plasmid

#89689

Purpose

pCAG promoter driving no CDS

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-N2c(G)

Plasmid

#73481

Purpose

N2c Glycoprotein expression vector

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

N2c(G)

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only