We narrowed to 59,355 results for: cloning

1. pcDNA5/FRT/TO GFP HSPA1L

   Plasmid

   #19484

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA1L (HSPA1L Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-H1-gRNA

   Plasmid

   #61089

   Purpose

   CRISPR/Cas9 system gRNA cloning

   Depositor

   Shenglin Huang

   Article

   Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

   Insert

   H1 promoter; gRNA sequences
   

   Use

   CRISPR; /cas9 grna cloning vector

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-U63-QtgRNA2.1-BR

   Plasmid

   #170513

   Purpose

   gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAJT194

   Plasmid

   #202293

   Purpose

   Type IIS cloning plasmid for LPattB5 integrations w/ OriT

   Depositor

   Christopher Voigt

   Article

   Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

   Insert

   sfGFP
   

   Expression

   Bacterial

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO DNAJB5

   Plasmid

   #19473

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB5 (DNAJB5 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBT100.106b

   Plasmid

   #122601

   Purpose

   clone APOBEC1 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   APOBEC1
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTEI036

   Plasmid

   #105350

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   LexA operator & 35S min promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCPP5154

   Plasmid

   #128704

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   avrPto
   

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT13-splitC

   Plasmid

   #122596

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CT3G-ERP2-MG

    Plasmid

    #175501

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP5616

    Plasmid

    #128727

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    avrPtoB
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCPP5534

    Plasmid

    #128723

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopX1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2pA2-mnx1:MLS-KillerRed

    Plasmid

    #115515

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Promoter

    -3mnx1

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDM026

    Plasmid

    #216808

    Purpose

    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Type

    Empty backbone

    Use

    CRISPR; Fungal expression

    Expression

    Bacterial

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQTEV-FLJ30046

    Plasmid

    #34828

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    FLJ30046 (SLAIN1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRII-1.1-1.10a

    Plasmid

    #69589

    Purpose

    OT-II TCR a chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR alpha clone 1.1 (Tcra Mouse)
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDONR221_SLC22A1

    Plasmid

    #132034

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A1 (SLC22A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAJT195

    Plasmid

    #202294

    Purpose

    Type IIS cloning plasmid for LPattB7 integrations w/ OriT

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    sfGFP
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYSD313

    Plasmid

    #212931

    Purpose

    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    Sed1 (SED1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-T3G-CERP2

    Plasmid

    #175498

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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