We narrowed to 19,506 results for: ACE;

1. hCD12

   Plasmid

   #225541

   Purpose

   Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

   Insert

   Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL11059

   Plasmid

   #68263

   Purpose

   Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMOD_B2101

   Plasmid

   #91060

   Purpose

   Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   35S

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMOD_B2112

   Plasmid

   #91064

   Purpose

   Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   PvUbi1

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMOD_B2203

   Plasmid

   #91066

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMOD_B2103b

   Plasmid

   #91062

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-N-mSOG-FRB-IRES-mCherry

   Plasmid

   #136602

   Purpose

   Encodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   N-mSOG-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMOD_B2312

   Plasmid

   #91070

   Purpose

   Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   PvUbi1

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

   Plasmid

   #136603

   Purpose

   Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   FKBP-C-mSOG-J-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMOD_B2301

    Plasmid

    #91067

    Purpose

    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNSSCPG

    Plasmid

    #183187

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMOD_B2309

    Plasmid

    #91069

    Purpose

    Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    YAO

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-bJun-C-mSOG-IRES-mCherry

    Plasmid

    #136600

    Purpose

    Encodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    bJun-C-mSOG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPbuCas13b-gRNA-NT

    Plasmid

    #184568

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-N-mSOG-bFos-IRES-mCherry

    Plasmid

    #136599

    Purpose

    Encodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    N-mSOG-bFos
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid

    #163930

    Purpose

    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD_B2121

    Plasmid

    #91065

    Purpose

    Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    GmUbi

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_B2403

    Plasmid

    #91071

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPgiCas13b-gRNA-1

    Plasmid

    #184834

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPgiCas13b-gRNA-NT

    Plasmid

    #184835

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPbuCas13b-gRNA-1

    Plasmid

    #184567

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HDAC4 Flag

    Plasmid

    #13821

    Purpose

    Mammalian expression of human histone deacetylase 4 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC4 (HDAC4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Sema6b(L)-AP-His

    Plasmid

    #72039

    Purpose

    Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6b (Sema6b Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Sema6b(S)-AP-His

    Plasmid

    #72040

    Purpose

    Expresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6b (Sema6b Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCSN067

    Plasmid

    #101748

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRMC2-tat:msfGFP

    Plasmid

    #194915

    Purpose

    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRMC2-sec:msfGFP

    Plasmid

    #194914

    Purpose

    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICSL11056

    Plasmid

    #68258

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMOD_C2200

    Plasmid

    #91082

    Purpose

    Module C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    none (will be fused to gRNA array in MODULE B)

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNSSCP

    Plasmid

    #183188

    Purpose

    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

    Depositor

    Hsiao-Chun Huang

    Article

    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

    Inserts

    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge