We narrowed to 40,826 results for: lat
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Plasmid#184750PurposeBig-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.DepositorInsertpEF1a-eGFP-T2A-BSD-bGHpA
UseCre/Lox, Mouse Targeting, and Synthetic Biology ;…ExpressionMammalianPromoterEF1aAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FGFR2-Fv-Fvls-E
Plasmid#15286DepositorInsertFGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)
TagsMyristoylation-targeting domain c-Src (14aa) and …ExpressionMammalianMutationFGFR2 cytoplasmic domain FKBP12 F36VAvailable SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 ORF3A_nostop
Plasmid#149319PurposeGateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 M_nostop
Plasmid#149322PurposeGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertM (M SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 ORF7A_nostop
Plasmid#149324PurposeGateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107576PurposeB. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic BiologyTagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterpdh promoter Bacillus megaterium DSM319Available SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-G6
Plasmid#73437PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.DepositorInsertRepressor G6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP5 Cys146Ala
Plasmid#154028PurposeGateway-compatible Entry vectorDepositorInsertNSP5
UseGateway-compatible entry vector, with mutagenized…MutationMany synonymous changes due to codon optimizationAvailable SinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 NSP5 Cys146Ala_nostop
Plasmid#154030PurposeGateway-compatible Entry vectorDepositorInsertNSP5
UseGateway-compatible entry vector, with mutagenized…MutationMany synonymous changes due to codon optimizationAvailable SinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pdCas9-M-3A2
Plasmid#73435PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.DepositorInsertRepressor 3A2 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 ORF9B_nostop
Plasmid#149327PurposeGateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF9B
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSH1/M-FGFR3-Fv-Fvls-E
Plasmid#15287DepositorInsertFGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
TagsMyristoylation-targeting domain c-Src (14aa) and …ExpressionMammalianMutationFGFR3 cytoplasmic domain only FKBP12 F36VAvailable SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSico Dnmt1
Plasmid#12165PurposeCre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating Dnmt1 shRNA expression.DepositorInsertDnmt1 shRNA (Dnmt1 Mouse)
UseCre/Lox, Lentiviral, and RNAiExpressionMammalianMutationEGFP is expressed from this plasmid as a marker, …Available SinceJune 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)
Plasmid#129028Purposeinactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNADepositorInsertdCas9-hudTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9-M-4A6
Plasmid#73434PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.DepositorInsertRepressor 4A6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBactin-td-mCherry-Cdk5rap2
Plasmid#196859PurposeExpression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.DepositorInserttd-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-KLF6-4D (1054)
Plasmid#49490Purposeexpresses human Flag tagged KLF6 with 4D mutationDepositorInsertKFL6-4D (KLF6 Human)
TagsFlagExpressionMammalianMutationthree serines and one tyrosine mutated to aspart…PromoterCMVAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH
Plasmid#112212PurposeTargeted DNA methylationDepositorAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
JA412
Plasmid#49935PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only