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pEJS2703-CMV-iNme2Smu-ABE8e-nt

Plasmid

#228724

Purpose

Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with iNme2Smu nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCardinal-Lysosomes-20

Plasmid

#56166

Purpose

Localization: Lysosome Membrane, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

LAMP1

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMBP-HRV3C-AcrIF9

Plasmid

#141442

Purpose

Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

Depositor

Blake Wiedenheft

Article

Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

Insert

AcrIF9

Tags

HRV3C cleavage site and Maltose Binding Protein

Expression

Bacterial

Promoter

ptac

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

Plasmid

#194682

Purpose

CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDECKO_TFRC_C (with mCherry)

Plasmid

#72627

Purpose

Expresses two gRNAs targeting the TFRC promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward TFRC

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

Plasmid

#233366

Purpose

hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Clathrin-15

Plasmid

#58005

Purpose

Localization: Clathrin Vesicles, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Clathrin (CLTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

Plasmid

#194685

Purpose

CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Brn2.WPRE

Plasmid

#67294

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEJS887_pTetR-P2A-BFPnls/sgAlpha

Plasmid

#108651

Purpose

Alpha satellite repeats targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Alpha satellite repeats targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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EYFP-Mito-7

Plasmid

#56596

Purpose

Localization: Mitochondria, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mPA-GFP-Actin-7

Plasmid

#57120

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Actin (ACTB Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mRuby2-Tubulin-C-18

Plasmid

#55915

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD

Plasmid

#233363

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubule

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

Kinprola_PKA-mEGFP-MTBD

Tags

mEGFP-MTBD

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

Plasmid

#194690

Purpose

hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBS L30 Ruby3-Rab6A

Plasmid

#135653

Purpose

Rab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab6A

Tags

Ruby3

Expression

Mammalian

Promoter

L30

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(Q61L)

Plasmid

#22783

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (Q61L) (RAC1 Human, A. victoria)

Tags

CyPet

Expression

Mammalian

Mutation

Rac1 Q61L.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
mTagBFP2-MAPTau-N-10

Plasmid

#55312

Purpose

Localization: Microtubules, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MAPTau (MAPT Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
BacPAK Myc-S1

Plasmid

#19424

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Rpn2 (PSMD1, RPN2 Human)

Tags

His and Myc

Expression

Insect

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pETSUMO2_Vitiosangium-bGSDM-thrombin

Plasmid

#222099

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Vitiosangium-bGSDM-thrombin ORF

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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