We narrowed to 22,760 results for: his
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Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y
Plasmid#191459PurposeCompare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent proteinDepositorInsertmTurquoise2_T203Y
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-mCherry
Plasmid#191466PurposeCompare the intensity of mCherry to mTurquoise2 fluorescent proteinDepositorInsertmCherry
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-GFP-9S [rpoA]
Plasmid#176221PurposeExpression of GFP-9S [rpoA]DepositorInsertGFP-9S [rpoA]
UseSynthetic BiologyTagsHis-GFPPromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-Pep-H
Plasmid#185788PurposeEncodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-Pep4
TagsHis-tagExpressionBacterialPromoterT7Available SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKP1693
Plasmid#167595PurposeExpress FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.DepositorInsertFepA
ExpressionBacterialMutationHis6 tag inserted after residue 393 in the mature…Available SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB1
Plasmid#168800PurposeFluorogen activating protein (FAP) DiB1DepositorInsertFluorogen activating protein DiB1
TagsHis-tagExpressionBacterialPromoteraraBADAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30 W96A-GLTP
Plasmid#170734PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-wtBlc
Plasmid#168258PurposeN-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip proteinDepositorInsertN-fragment of wtBlc + Leucine Zipper
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB3
Plasmid#168802PurposeFluorogen activating protein (FAP) DiB3DepositorInsertFluorogen activating protein DiB3
TagsHis-tagExpressionBacterialPromoteraraBADAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3b
Plasmid#166054Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertOrbicella faveolata caspase-3b
TagsHis tagExpressionBacterialPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp3
Plasmid#166052Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertPorites astreiodes caspase-3
TagsHis tagExpressionBacterialPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL1965
Plasmid#155152PurposeExpresses MlaF(1-246)-(6 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1966
Plasmid#155153PurposeExpresses MlaF(1-246)-(10 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1967
Plasmid#155154PurposeExpresses MlaF(1-246)-(14 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-1
Plasmid#136385PurposeExpresses DANCER-1 dynamic proteinDepositorInsertDANCER-1
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - DANCER-2
Plasmid#136386PurposeExpresses DANCER-2 dynamic proteinDepositorInsertDANCER-2
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L5A-L7I-A34F-V39LPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ414 - NERD-S
Plasmid#136388PurposeExpresses NERD-S static proteinDepositorInsertNERD-S
Tags6x-His tag (N-term of insert)ExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V39I-V54IPromoterT7 promoterAvailable SinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz106_CsoS2_MR
Plasmid#140843PurposeHis-tagged CsoS2 Middle Region (MR), pET-14b backboneDepositorInsertCsoS2 Middle Region (MR)
TagsHexahistidine tagExpressionBacterialMutationonly amino acids 257-603PromoterT7Available SinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz109_CsoS2_NTD
Plasmid#140842PurposeHis-tagged CsoS2 N-terminal domain (NTD), pET-14b backboneDepositorInsertCsoS2 N-terminal domain (NTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 262-869PromoterT7Available SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz47_polyPro_CsoS2_Npeptide
Plasmid#140845PurposeConsensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backboneDepositorInsertpolyproline helix with CsoS2 N-peptide
TagsHexahistidine tag and Strep-tag IIExpressionBacterialPromoterT7Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
TagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-β20cp6
Plasmid#127060PurposeLow phosphate inducible circular permutation of GFP, which is a substrate of the lon proteaseDepositorInsertGFPcp6-beta20
TagsHis tagExpressionBacterialPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW084
Plasmid#134130PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
TagsGFPExpressionBacterialMutationR236AAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW095
Plasmid#134131PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
TagsGFPExpressionBacterialMutationR155AAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB HTB_hMSH6_T1219D
Plasmid#135466PurposeExpression human MSH6_T1219D mutant protein using baculovirusDepositorInserthuman MSH6_T1219D (MSH6 Human)
TagsHis-tagExpressionInsectMutationchanged threonine to aspartic acidAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u36_Snifit
Plasmid#119045PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)DepositorInsertMTX-u36_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation36TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u51_Snifit
Plasmid#119046PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)DepositorInsertMTX-u51_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation51TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u23_Snifit
Plasmid#119044PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)DepositorInsertMTX-u23_Snifit
TagsHis-tag and Strep-tagExpressionBacterialMutation23TAG R44L H45QPromoterpBADAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MtMscL delta49
Plasmid#118342PurposeM. tuberculosis MscL (delta49) in pET19bDepositorInsertMscL (mscL Mycobacterium tuberculosis)
Tags6x His Tag and Thrombin Cleavage siteExpressionBacterialMutationChanged Glutamic Acid (Glu) 102 to Stop CodonPromoterT7 PromoterAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only