59,544 results

1. pD649-HAsp-SIGLEC15-COMP5AP-AviTag-9xHis

   Plasmid

   #157262

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SIGLEC15 (SIGLEC15 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pk7-GR-GFP

   Plasmid

   #15534

   Depositor

   Ian Macara

   Article

   Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

   Insert

   Glucocorticoid receptor (NR3C1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The glucocorticoid receptor was cloned into the X…

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACE2

   Plasmid

   #164219

   Purpose

   Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Mutation

   silent mutation to remove internal EcoRI site (GA…

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SAP145

   Plasmid

   #11239

   Depositor

   Robin Reed

   Article

   Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

   Insert

   SAP145 (SF3B2 Human)
   

   Expression

   Bacterial

   Mutation

   First 7 amino acids are missing compared to NP_00…

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Active Wnt11-V5

   Plasmid

   #43824

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   human WNT11 (WNT11 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-EGFP-C1-HsDCP1a

   Plasmid

   #25030

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   DCP1a (DCP1A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. WT WWP1

   Plasmid

   #200085

   Purpose

   WWP1 in pQCXIN vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   WT WWP1 (WWP1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLKO.1-UPF1-CDS

   Plasmid

   #136037

   Purpose

   UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. R701-X65-527: His6-tev-Hs.CALM1(2-148)

   Plasmid

   #159693

   Purpose

   E. coli protein expression of His6-tev-Hs.CALM1(2-148)

   Depositor

   Dominic Esposito

   Article

   Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

   Insert

   His6-tev-Hs.CALM1(2-148) (CALM1 Human)
   

   Tags

   His6-tev

   Expression

   Bacterial

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0629

    Plasmid

    #142667

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1I2 (NR1I2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRT/TO/FLAG/HA-DEST PABPC4

    Plasmid

    #19882

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Poly(A) binding protein, cytoplasmic 4 (PABPC4 Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. CCR2-DuET

    Plasmid

    #213198

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR2 (CCR2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGateway 3XFlag Prickle1 flag tagged

    Plasmid

    #24644

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    Prickle 1 (PRICKLE1 Human)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Available Since

    April 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

14. NKP38 GFP (1009)

    Plasmid

    #62021

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    AYTL1 (LPCAT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. VPS29-YFP

    Plasmid

    #174520

    Purpose

    Expresses human VPS29 with a C-terminal YFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Rojas et al J Cell Biol. 2008 Nov 3;183(3):513-26. d

    Insert

    VPS29 (VPS29 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36

    Plasmid

    #111745

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q36 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCVL.SSA TLR (Sce)

    Plasmid

    #45576

    Purpose

    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-hPar2

    Plasmid

    #53228

    Purpose

    mammalian expression of human Par2

    Depositor

    Shaun Coughlin

    Article

    Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

    Insert

    Par2 (F2RL1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 c-Fyn

    Plasmid

    #16032

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. hTLR3-pcDNA3

    Plasmid

    #32712

    Depositor

    Saumen Sarkar

    Article

    Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

    Insert

    TLR3 (TLR3 Human)
    

    Tags

    FLAG and Preprototrypsin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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