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pLS415

Plasmid

#239126

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLS405_YCR051W

Plasmid

#239132

Purpose

Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Integrating

Expression

Bacterial and Yeast

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLS413

Plasmid

#239124

Purpose

Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker HIS3

Depositor

Sahand Jamal Rahi

Article

Scutteri et al Synth Biol (Oxf). 2025 May 21;10(1):ysaf

Type

Empty backbone

Use

Centromeric

Expression

Bacterial and Yeast

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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p365_DG

Plasmid

#234688

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and ALFA

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

ALFA and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLS400

Plasmid

#231080

Purpose

Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX

Depositor

Sahand Jamal Rahi

Article

Scutteri et al bioRxiv 2024.11.05.622133

Type

Empty backbone

Use

Integrating

Expression

Bacterial and Yeast

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pVP311

Plasmid

#222070

Purpose

MultiGreen 2.0 AB level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP312

Plasmid

#222071

Purpose

MultiGreen 2.0 BC level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP313

Plasmid

#222072

Purpose

MultiGreen 2.0 CD level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP314

Plasmid

#222073

Purpose

MultiGreen 2.0 DE level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP315

Plasmid

#222074

Purpose

MultiGreen 2.0 EF level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP316

Plasmid

#222075

Purpose

MultiGreen 2.0 FG level 1 acceptor vector, kanamycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP078

Plasmid

#222030

Purpose

MultiGreen 2.0 AB level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP079

Plasmid

#222031

Purpose

MultiGreen 2.0 BC level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP080

Plasmid

#222032

Purpose

MultiGreen 2.0 CD level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP081

Plasmid

#222033

Purpose

MultiGreen 2.0 DE level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP082

Plasmid

#222034

Purpose

MultiGreen 2.0 EF level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVP083

Plasmid

#222035

Purpose

MultiGreen 2.0 FG level 1 acceptor vector, spectinomycin selection.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All unused Esp3i sites are domesticated

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
insulator_seq_barcode_vector

Plasmid

#221458

Purpose

Vector for generating a barcode library for insulator-seq.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Type

Empty backbone

Use

Synthetic Biology and Unspecified

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBH111

Plasmid

#215778

Purpose

NBU2 backbone with anti-LacI(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-LacI(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0

Plasmid

#196495

Purpose

CRISPR tool to target astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pETURALEU

Plasmid

#223207

Purpose

Plasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markers

Depositor

Sahand Jamal Rahi

Article

Stojkovic et al G3 (Bethesda). 2024 Dec 17:jkae297. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBH102

Plasmid

#215773

Purpose

NBU2 backbone with LacI(TAN) and P(tta)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(TAN)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(tta)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH103

Plasmid

#215774

Purpose

NBU2 backbone with LacI(KSL) and P(agg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(KSL)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(agg)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH104

Plasmid

#215775

Purpose

NBU2 backbone with LacI(HQN) and P(ttg)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(HQN)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(ttg)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBH105

Plasmid

#215776

Purpose

NBU2 backbone with LacI(GKR) and P(gac)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

LacI(GKR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(gac)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBH116

Plasmid

#215779

Purpose

NBU2 backbone with anti-RbsR(YQR) and P(O1)_nanoluc

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Inserts

anti-RbsR(YQR)
Nanoluc

Use

Synthetic Biology

Expression

Bacterial

Promoter

Bt1311 and P(O1)

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBH501

Plasmid

#215780

Purpose

NBU1 backbone with constitutive dCas9

Depositor

Corey Wilson

Article

Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

Insert

dCas9

Use

Synthetic Biology

Expression

Bacterial

Promoter

P1

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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RT-1

Plasmid

#220434

Purpose

Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
T8-pAc5.1 lambdaN HA EcoRV

Plasmid

#206492

Purpose

Schneider cell imaging

Depositor

Anne Ephrussi

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Expression

Insect

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMYT082

Plasmid

#198864

Purpose

Markerless yeast integration Vector at Int.8 locus to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge