We narrowed to 77,445 results for: Mycs;

1. attB53-Pac-attB53

   Plasmid

   #183610

   Purpose

   RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   Pac
   

   Use

   Rmce

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRMTK-clo10

   Plasmid

   #203961

   Purpose

   Cloning plasmid containing the erythromycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Erythromycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   N/A

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGH156_MS2-AID-Hygro

   Plasmid

   #85415

   Purpose

   lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   MS2-AID and Hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT85_P438-mEOS3.2_GentaR

   Plasmid

   #173894

   Purpose

   Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

   Depositor

   Yonathan Arfi

   Article

   Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

   Insert

   mEos3.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P438

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b-dspB(E184Q)-6xHis

   Plasmid

   #175802

   Purpose

   Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAC690-TEF

   Plasmid

   #160432

   Purpose

   Expresses eGFP under control of the yeast TEF2 promoter

   Depositor

   Gary Stormo

   Article

   Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

   Insert

   S. cerevisiae TEF2 promoter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   S. cerevisiae TEF2

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

   Plasmid

   #58441

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR L1-vas2-Cre-L4

   Plasmid

   #62301

   Purpose

   Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Insert

   Cre recombinase under control of vasa promoter and terminator
   

   Use

   Tags

   HA and SV40 NLS

   Expression

   Insect

   Mutation

   Promoter

   vasa

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTopo_Ex2Donor_pPGK-PURO

   Plasmid

   #198862

   Purpose

   Donor template for CRISPR/Cas9 targeting of TERT Ex2

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   PURO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKMV-VioC

    Plasmid

    #65349

    Purpose

    systhesis of vioC gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-sCS4-2T

    Plasmid

    #28250

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain Cse4-Scm3-H4 with 2 Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between Cse4 and Scm3. An…

    Promoter

    Available since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pME-nlsGal80(opt)

    Plasmid

    #51618

    Purpose

    vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80(opt) (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    NLS

    Expression

    Mutation

    codon optimized

    Promoter

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUM31dao

    Plasmid

    #87242

    Purpose

    Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

    Depositor

    Anil Day

    Article

    Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

    Insert

    D-amino oxidase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    plastid rrn

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKMV-VioE

    Plasmid

    #65351

    Purpose

    systhesis of vioE gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Lyn-AktAR

    Plasmid

    #123356

    Purpose

    FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    Lyn-AktAR
    

    Use

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUltra-5HTP

    Plasmid

    #178042

    Purpose

    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T107C, P254T, C255A

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pILGFPQ4

    Plasmid

    #83559

    Purpose

    Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL7 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKMV-VioA

    Plasmid

    #65347

    Purpose

    systhesis of vioA gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH-macroHC

    Plasmid

    #82337

    Purpose

    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli

    Depositor

    Michael Hothorn

    Article

    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p

    Insert

    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    

    Use

    Tags

    6xHis non-cleavable and macro domain of human his…

    Expression

    Bacterial

    Mutation

    Truncated protein, amino acids 1-178

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

    Plasmid

    #188704

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    NFATC2 sgRNAs
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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