We narrowed to 14,023 results for: egf

1. pAc5.1B-EGFP-DmTral-LSm-S13AI15A-V5His6_C

   Plasmid

   #146055

   Purpose

   Insect Expression of DmTral-LSm-S13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-S13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation A198C compared to the sequenc…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C

   Plasmid

   #146046

   Purpose

   Insect Expression of DmTral-LSm-del46-60

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSm-del46-60 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C

   Plasmid

   #146006

   Purpose

   Insect Expression of DmSmaug-del584-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug-del584-762 (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   7 silent mutations and a deletion of the AA 961-9…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

   Plasmid

   #176573

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

   Plasmid

   #175256

   Purpose

   SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

   Depositor

   Johanna Ivaska

   Article

   Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

   Insert

   SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

   Plasmid

   #146049

   Purpose

   Insect Expression of DmTral-LSmR21EE23KS13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KS13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   S13A, I15A, R21E, E23K, one silent mutation A198C…

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. C512-E01: attB5r-meGFP-nostop-attB1r

   Plasmid

   #162940

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric enhanced Green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

   Plasmid

   #167478

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(I539E)-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

   Plasmid

   #167479

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(C450A)-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Mm_Plk5(300-595) PBD domain

    Plasmid

    #136316

    Purpose

    Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fragment 300-595

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP(1-233)-mApple(2-236)-HRas(2-189)

    Plasmid

    #133855

    Purpose

    Mammalian expression of HRas N-terminally tagged with EGFP and mApple

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    HRas (HRAS Human)
    

    Use

    Tags

    EGFP and mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid

    #131133

    Purpose

    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

    Depositor

    Ralf Jansen

    Insert

    NLS-HA-2xMCP-EGFP-cMycBirA*
    

    Use

    Lentiviral

    Tags

    NLS, HA, cMyc (N-terminal of BirA*)

    Expression

    Mammalian

    Mutation

    BirA* = BIrA >R118G

    Promoter

    human ubiquitin C (UBC)

    Available Since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid

    #121803

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + EF1a::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121807

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid

    #121748

    Purpose

    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    OLLAS epitope tag-IRES2-eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-N1 Dynamin1 T78R L84R T92R V118R

    Plasmid

    #112106

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T78R L84R T92R V118R

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEST-p15A-Pl-lacO1-deGFP-T500 (123p)

    Plasmid

    #70199

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEST-p15A-J23113-deGFP-T500 (124p)

    Plasmid

    #70200

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge