We narrowed to 9,345 results for: sacs

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pDY-HA1-HA2-neo

Plasmid

#109123

Purpose

FR cassette provider to introduce FR cassette to a BAC by recombineering

Depositor

David Gilbert

Article

Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

Inserts

HA1
HA2

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

Plasmid

#122067

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUAST mito roGFP2-Orp1

Plasmid

#64997

Purpose

for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET-HJURP-CBP

Plasmid

#30393

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

HJURP CenpA Binding Domain (CENPA Human, Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGST-TEV-yVms1(1-417,delta(38-69))

Plasmid

#128739

Purpose

Bacterial overexpression and crystallization

Depositor

Chris Hill

Article

Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

Insert

Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

residues 1-417 with deletion of 38-69

Promoter

T7

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.MYB

Plasmid

#184660

Purpose

Retroviral expression of mouse RAP1 delta MYB

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta MYB

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.COIL

Plasmid

#184659

Purpose

Retroviral expression of mouse RAP1 delta COIL

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

pLPC

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta COIL

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.BRCT

Plasmid

#184658

Purpose

Retroviral expression of mouse Rap1 delta BRCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

RAP1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta BRCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.RCT

Plasmid

#184661

Purpose

Retroviral expression of mouse RAP1 delta RCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta RCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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IpO

Plasmid

#48233

Purpose

Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.

Depositor

Ronald Davis

Article

Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

Inserts

URA3 3' fragment
URA3 5' fragment

Use

Routine cloning vector

Mutation

-242 upstream to URA3 ORF +495 and URA3 ORF from …

Promoter

URA3 promoter and none

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pFS485

Plasmid

#89348

Purpose

Leu1 integrating plasmid with cdc25 genomic fragment

Depositor

Nick Rhind

Article

Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

Insert

cdc25 (cdc25 Fission Yeast)

Expression

Yeast

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1-GFP

Plasmid

#39353

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

GFP and kanMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
Sha2Cas9-HF

Plasmid

#192142

Purpose

Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9-HF

Use

AAV

Expression

Mammalian

Mutation

Sha2Cas9-HF (R247A and N415A)

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpeCas9

Plasmid

#192139

Purpose

Expresses SpeCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

SpeCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pNB813

Plasmid

#60161

Purpose

Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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XW20

Plasmid

#65836

Purpose

Punc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

OR74A quinic acid utilization activator (qa-1F)

Expression

Worm

Mutation

The endogenous SphI, KpnI and SacI sites in QF ar…

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17

Plasmid

#121394

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Promoter

ADH1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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