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pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Publication

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Designed VEGF-binding scFv G6des13

Plasmid

#110213

Purpose

Yeast Surface Display of G6des13

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des13

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSc-His6-Sem1Sc

Plasmid

#83235

Purpose

pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

Depositor

Gali Prag

Publication

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Sem 1 (SEM1 Budding Yeast)

Tags

His6

Expression

Yeast

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pYDS2.0_AT118-L

Plasmid

#222847

Purpose

Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Publication

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Expression

Yeast

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pXNgate22-1HA

Plasmid

#105077

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Leu2 drive

Plasmid

#89578

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Depositor

George Church

Publication

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Leu2 gene drive

Expression

Yeast

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan-ADE2

Plasmid

#232105

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Nat-ADE2

Plasmid

#232103

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Hyg

Plasmid

#232098

Purpose

Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

Depositor

Jeffrey Lewis

Publication

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-VSV

Plasmid

#212752

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Publication

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTSK573

Plasmid

#190881

Purpose

For C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiae

Depositor

Tatiana Karpova, Gunjan Mehta

Publication

Podh et al STAR Protoc. 2022 Dec 16;3(4):101900. do

Insert

HaloTag

Use

Bacterial cloning vector

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGBKT7-RmMYB108

Plasmid

#173183

Purpose

Expresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.

Depositor

Daidi Che

Publication

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Expression

Yeast

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pfa6a mScarlet-FKBP::KANMX

Plasmid

#185905

Purpose

Plasmid for cloning mScarlet fused to FKBP into yeast.

Depositor

Matthew Good

Publication

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-FKBP

Tags

mScarlet fused to FKBP

Expression

Yeast

Promoter

SP6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRS405 SEC66-VAC8-RFP

Plasmid

#166843

Purpose

Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, SEC66 Budding Yeast)

Tags

RFP and SEC66 transmembrane domain

Expression

Yeast

Mutation

SEC66 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3487 (YCp LEU2 Rpb1 S1493A)

Plasmid

#91809

Purpose

Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed serine 1493 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3488 (YCp LEU2 Rpb1 P1494A)

Plasmid

#91810

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed proline 1494 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3508 (YCp LEU2 Rpb1 Y1473A)

Plasmid

#91811

Purpose

Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed tyrosine 1473 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3509 (YCp LEU2 Rpb1 T1471A)

Plasmid

#91812

Purpose

Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed threonine 1471 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3511 (YCp LEU2 Rpb1 P1472A)

Plasmid

#91813

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed proline 1472 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRS313-PRP8

Plasmid

#90964

Purpose

yeast vector

Depositor

Christine Guthrie

Publication

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Promoter

native

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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