We narrowed to 10,413 results for: plasmids 101

1. (SUMO)10-(SIM)5

   Plasmid

   #126866

   Purpose

   Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LZF1735 pAAV_hSyn-DiO-somArchon1-EGFP-P2A-somCheRiff

   Plasmid

   #126512

   Purpose

   Optopatch4. Simultaneous optical recording and manipulation of electrical activity in neurons

   Depositor

   Adam Cohen

   Article

   Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

   Insert

   somArchon1-EGFP-P2A-somCheRiff
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-Puro-TNNT2-Neo

   Plasmid

   #214013

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. anti-Notch2_B9-pBIOCAM5

   Plasmid

   #39348

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch2 (clone B9) (NOTCH2, Notch2 Human, Mouse)
   

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP1594_2x_NLS

   Plasmid

   #110625

   Purpose

   Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   None
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-human NMyc-humanER-IRES-mCherry

   Plasmid

   #73571

   Purpose

   Mammalian retroviral expression of NMyc-ER

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   NMyc (MYCN Human)
   

   Use

   Retroviral

   Tags

   Human ER and IRES-mCherry

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAGH10 - RlucFluc minus 1 HIV-1

   Plasmid

   #198224

   Purpose

   Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

   Depositor

   Isha Jain

   Article

   Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

   Inserts

   Renilla luciferase
   Firefly luciferase
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

   Plasmid

   #187358

   Purpose

   Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   HPC-4 antibody heavy chain, and 6xHIS tag
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-hCMV1-hNANOG-IV

   Plasmid

   #60432

   Purpose

   tet-inducible human NANOG

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   NANOG (NANOG Human)
   

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgRGFP

    Plasmid

    #82695

    Purpose

    Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    PGK, U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

    Plasmid

    #83489

    Purpose

    cre-dependent PAK1-AID under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    PAK1-AID-ires-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

    Plasmid

    #105039

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-HCF-1rep1E10A

    Plasmid

    #84607

    Purpose

    Plasmid contains HCF-1rep1E10A with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1E10A (HCFC1 Human)
    

    Tags

    GST and His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-HCF-1rep1wt

    Plasmid

    #84606

    Purpose

    Plasmid contains HCF-1rep1wt with GST-tag

    Depositor

    Winship Herr

    Article

    Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

    Insert

    HCF-1rep1wt (HCFC1 Human)
    

    Tags

    GST-TEV and His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CAG-FLEX-HA-DLX2-T2A-mCherry

    Plasmid

    #178585

    Purpose

    Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    DLX2 (DLX2 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-REPORT (PGK) 5xGRE-NanoLuc

    Plasmid

    #207170

    Purpose

    GAl4 response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

    Inserts

    NanoLuciferase
    d2eGFP
    5x GRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Promoter

    Gal4 response element minP and PGK

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. tetO-SMARCD3

    Plasmid

    #170701

    Purpose

    doxycycline-inducible overexpression of SMARCD3

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SMARCD3 (SMARCD3 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAVS1-Neo-TNNT2-Puro

    Plasmid

    #214016

    Purpose

    Expression of cardiac troponin T

    Depositor

    Paul Burridge

    Article

    Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

    Insert

    TNNT2 (TNNT2 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge