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BIN1-pmCherryN1

Plasmid

#27693

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

BIN1 (Bin1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_mNeongreen

Plasmid

#182413

Purpose

Bacterial expression of mNeongreen

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LaM8-GG15-irFP

Plasmid

#159591

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Use

Lentiviral

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseA-6xHis

Plasmid

#214808

Purpose

Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease A

Tags

6xhis

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-R5-GFP

Plasmid

#17838

Depositor

Jay Keasling

Article

Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

Insert

pET30-GFP

Use

Synthetic Biology

Tags

His, R5, and S-tag

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR1K18ms

Plasmid

#72644

Purpose

Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Article

Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available Since

March 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHCMV-RabiesG

Plasmid

#15785

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Rabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pG1

Plasmid

#162602

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-6xHis

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pT2SC

Plasmid

#59382

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1

Plasmid

#58434

Purpose

Induces Citrine expression from a promoter containing 4 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)4-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.4

Plasmid

#235055

Purpose

Expresses Tad-CBE2.4 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.4-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox_v2

Plasmid

#198040

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKG1334_pHarbor-PiggyBac_backbone

Plasmid

#239705

Purpose

PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integration

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-rm_gCarvi-T2A

Plasmid

#190334

Purpose

Ratiometric reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

ratiometric gCarvi

Use

Unspecified

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-HygroR

Plasmid

#207177

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_Uricase

Plasmid

#182400

Purpose

Bacterial expression of uricase

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

uricase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5CA (Q79L)

Plasmid

#205705

Purpose

Mammalian expression of BFP-tagged mutant Rab5CA

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

RAB5A (RAB5A Human)

Expression

Mammalian

Mutation

Q79L

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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paavCAG-Jx-rev-post-mGRASP-2A-dTomato

Plasmid

#34913

Purpose

JxON-post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

postsynaptic mGRASP

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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