Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,071 results for: sgRNA

Clear keywords
Showing: 6661 - 6680 of 9071 results

  2. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

    Enlarge

  3. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

    Enlarge

  6. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

    Enlarge

  14. px330 VRER

    Plasmid
    #101729
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VRER
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101729-plasmid-map-sequence-id-265859

    Enlarge

  17. pNSU299_YDR344C

    Plasmid
    #166072
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166072-plasmid-map-sequence-id-329603

    Enlarge

  19. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid
    #126706
    Purpose
    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence
    Depositor
    Darrell Kotton
    Publication
    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    Use
    CRISPR
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126706-plasmid-map-sequence-id-245754

    Enlarge

Showing: 6661 - 6680 of 9071 results