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LZRS-hNEDD9_IresGFP

Plasmid

#21962

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

NEDD9 (NEDD9 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
His-GFP-FCHo2

Plasmid

#170870

Purpose

Mammalian expresssion of His-GFP-FCHo2.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

FCH domain only 2 (Fcho2) (Fcho2 Mouse)

Tags

(His)6 and mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

Plasmid

#90009

Purpose

Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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CMV10-3xFlag Skp2 WT

Plasmid

#81115

Purpose

mammalian expression of Skp2 WT

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Caveolin-3

Plasmid

#68396

Purpose

Fluorescently tagged Caveolin-3 for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
EYFP-JAB1

Plasmid

#111213

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Orel et al J Cell Mol Med. 2010 Jun;14(6B):1555-68.

Insert

CSN5 (COPS5 Human)

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-attB-PUb-GFP

Plasmid

#183911

Purpose

attB-site containing docking plasmid for genomic insertion into attP site, encoding GFP under control of the Aedes aegypti PUb promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Promoter

Aedes aegypti PUb

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGH00.0126

Plasmid

#64257

Purpose

Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

Depositor

Mark Guiltinan, Siela Maximova

Article

Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

Inserts

EGFP
neomycin phosphotransferase II

Use

Binary agrobacterium vector for plant transformat…

Expression

Plant

Promoter

E12-omega

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pEF1a-tTAc

Plasmid

#172123

Purpose

Constitutive expression of InteinC-tTAC

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

InteinC-tTAc

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST12.2 DYRK2 WT

Plasmid

#20005

Depositor

Anjana Rao

Article

Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

Insert

DYRK2 (DYRK2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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tdTomato-Gamma-Tubulin-17

Plasmid

#58099

Purpose

Localization: Centrosomes, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pRcCMV myc-Plk1 wt (Nigg RG6)

Plasmid

#41160

Depositor

Erich Nigg

Article

Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

Insert

Plk1 (PLK1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shIFNAR

Plasmid

#127699

Purpose

Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shIFNAR1 Mmu (Ifnar1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDrVSP(C302S)-IRES2-EGFP

Plasmid

#80331

Purpose

Expresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cell

Depositor

Yasushi Okamura

Article

Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

Insert

Daino rerio voltage-sensing phosphatase (tpte Zebrafish)

Expression

Mammalian

Mutation

C302S

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSCALPS-FLAG-HA-mmuMettl3 APPA

Plasmid

#207124

Purpose

Lentiviral plasmid to express mouse Mettl3 APPA mutant

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA

Mutation

D395A and W398A

Promoter

SSFV

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCPΔCR

Plasmid

#83239

Purpose

The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCpdCr

Tags

roGFP2 fusion with Tsa2dCpdCr

Expression

Yeast

Promoter

TEF

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-Spo20(PABD)

Plasmid

#221758

Purpose

Stable expression of GFP-Spo20(PABD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Spo20 (SOS1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem Spo20(PABD) (amino acids 422-551)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Stat3 S727A pRc/CMV

Plasmid

#8708

Depositor

Jim Darnell

Article

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 S727A (Stat3 Mouse)

Expression

Mammalian

Mutation

S727A (exhibits o serine phosphorylation either c…

Available Since

Aug. 11, 2005

Availability

Academic Institutions and Nonprofits only

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