We narrowed to 55,065 results for: ran

1. pSpCas9_T2A_mCherry sgAAVS1

   Plasmid

   #192480

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HIV-1-GFP ΔEnv

   Plasmid

   #217437

   Purpose

   HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   gag/pol
   

   Use

   Lentiviral

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTmpl-miniDonor-GFP

   Plasmid

   #238107

   Purpose

   Mini donor expressing GFP, used for PCR to generate IVT template.

   Depositor

   Feng Zhang

   Article

   Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

   Insert

   CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence
   

   Expression

   Mammalian

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHJ12: CadC-VHH-Caffeine (No linker)

   Plasmid

   #108244

   Purpose

   Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
   

   Use

   Synthetic Biology

   Promoter

   pLacO1

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-SAM

   Plasmid

   #102559

   Purpose

   Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Inserts

   MS2-P65-HSF1_T2A_Hygro
   dCAS9(D10A, N863A)-VP64_T2A_Blast
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9 and N55K in MS2

   Promoter

   CAG and EF1A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)mGAT1-0-GFP

   Plasmid

   #41662

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal hydrophobic isoleucine residue added a…

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. JN202: pMVP/SB/Blast-DEST

   Plasmid

   #121864

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNvT-MHC::mCh

   Plasmid

   #67943

   Purpose

   Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

   Depositor

   Ulrich Technau

   Article

   Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   mCherry
   

   Use

   Transgenic reporter in the sea anemone, nematoste…

   Promoter

   Myosin Heavy Chain1 (MyHC1)

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET24a-VHH-tev-mCherry

    Plasmid

    #117752

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. opto-Cdc42

    Plasmid

    #174510

    Purpose

    Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Cdc42-mCherry
    

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-Bxb1-LP-TC

    Plasmid

    #183754

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    loxP-eGFP-attP-Bsd-lox257
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Rab8a-wt

    Plasmid

    #86075

    Purpose

    A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. fwYellow chromoprotein

    Plasmid

    #117841

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, fwYellow
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. tsPurple chromoprotein

    Plasmid

    #117848

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEHIShqprt

    Plasmid

    #92166

    Purpose

    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    Quinolinate Phosphoribosyltransferase (QPRT Human)
    

    Tags

    TEV protease -cleavable N-terminal 6His

    Expression

    Bacterial

    Mutation

    changed Asp 2 to Asn to create BspHI site

    Promoter

    T7

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tol2-CAG::Cytbow

    Plasmid

    #158991

    Purpose

    Multicolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Cytbow
    

    Tags

    The default expressed EBFP2 is fused to H2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-SpCas9-NG

    Plasmid

    #117919

    Purpose

    Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9_T2A_mCherry sgBFP

    Plasmid

    #192929

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge