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p35S:Citrine-TurboID-3xHA

Plasmid

#199243

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID

Tags

3xHA and TurboID-3xHA

Expression

Plant

Promoter

CaMV 35S

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMK291 (mAID-mClover-Bsr)

Plasmid

#72829

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Bsr

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22D

Plasmid

#91136

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pB[vasa-Cas9 PUb-ZsGreen]

Plasmid

#169012

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster vasa

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon2

Plasmid

#195526

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAB1678 CMV-HIVNES-GS-Cas13bt1

Plasmid

#176316

Purpose

CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt1

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Expression

Yeast

Promoter

tetO7 promoter

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD(V203D)

Plasmid

#190090

Purpose

Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD(V203D)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMK294 (mAID-mCherry2-Bsr)

Plasmid

#72832

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Bsr

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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