We narrowed to 13,271 results for: cDNA

1. pAL189

   Plasmid

   #22278

   Purpose

   Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cenp-B DBD INCENP mCherry

   Plasmid

   #45233

   Purpose

   Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B-DBD INCENP-Δcen mCherry
   

   Use

   Tags

   DNA binding domain of CENP-B and mCherry

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVH72-Tier1-PhCMV-SEAP

   Plasmid

   #169560

   Purpose

   Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven human secreted placental alkaline phosphatase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. human syndecan 2 EFYA

   Plasmid

   #64971

   Purpose

   Constitutive expression in mammalian cells

   Depositor

   Enric Espel

   Article

   Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

   Insert

   syndecan 2 (SDC2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted EFYA

   Promoter

   CMV

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. human syndecan 2 FAAF

   Plasmid

   #64972

   Purpose

   Constitutive expression in mammalian cells

   Depositor

   Enric Espel

   Article

   Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

   Insert

   syndecan 2 (SDC2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y179F, S187A, S188A, Y191F

   Promoter

   CMV

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. aq-CFP

   Plasmid

   #36073

   Depositor

   Narasimhan Gautam

   Article

   Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

   Insert

   G protein subunit alpha q (Gnaq Mouse)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP inserted between amino acid 124 and 125

   Promoter

   CMV

   Available Since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

7. Lyn-FRB-GFP11

   Plasmid

   #181863

   Purpose

   Plasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   Lyn-FRB-GFP11
   

   Use

   Tags

   GFP11 and N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. L1-A426D

   Plasmid

   #15485

   Depositor

   Vance Lemmon

   Article

   Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

   Insert

   L1-A426D (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Alanine 426 changed to Aspartic Acid

   Promoter

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

9. Cav1-mRed

   Plasmid

   #12681

   Depositor

   Richard Pagano

   Article

   Sharma et al Mol Biol Cell. 2004 Jul . 15(7):3114-22.

   Insert

   canine caveolin-1 (CAV1 Canis familiaris)
   

   Use

   Tags

   mRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

10. ATF4 18: RLuc.5’ATF4.GFP

    Plasmid

    #21864

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

11. Ilmol

    Plasmid

    #120310

    Purpose

    A red-shifted GEVI consists of triple mutant ciona based voltage sensing domain with optimized linker and dTomato K159R.

    Depositor

    Bradley Baker

    Article

    Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

    Insert

    Ilmol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPD385 VPR-ZF43(RAAA)

    Plasmid

    #138824

    Purpose

    Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43(RAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R15A, R43A, R71A

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGas-miRFP670nano3

    Plasmid

    #184672

    Purpose

    G protein alpha-subunit internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    G protein alpha-s-miRFP670nano3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. iBlueberry T2A mCherry

    Plasmid

    #72434

    Purpose

    Expressing of iBlueberry and mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

    Insert

    iBlueberry T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutation E177Q in iBlueberry

    Promoter

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pFL-V5-GW

    Plasmid

    #78857

    Purpose

    DULIP Prey Destination Vector (Gateway, N-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPA-RL-GW

    Plasmid

    #78855

    Purpose

    DULIP Bait Destination Vector (Gateway, N-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Protein A-Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA PRAS40 S183A

    Plasmid

    #15674

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 S183A (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S183A

    Promoter

    Available Since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

19. 2xFRB-myc

    Plasmid

    #20227

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pId1-IRES-YFP

    Plasmid

    #22667

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Inhibitor of DNA binding 1 (Id1 Mouse)
    

    Use

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. h-alpha-ENaC-2

    Plasmid

    #83431

    Purpose

    full length human alpha ENaC-2 with flag tag

    Depositor

    Christie Thomas

    Article

    Thomas et al Am J Physiol. 1998 May;274(5 Pt 1):C1312

    Insert

    human alpha ENaC isoform 2 (SCNN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. V5-WT-short-UBASH3A

    Plasmid

    #192088

    Purpose

    Mammalian expression of human wild-type short-form UBASH3A with V5 tag

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tau exon 9–12 FTDP-17 N279K splicing minigene

    Plasmid

    #122967

    Purpose

    expresses circular tau RNAs with the FTDP-17 N279K

    Depositor

    Stefan Stamm

    Article

    Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

    Insert

    maps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FTDP-17 mutation

    Promoter

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pdCas9-sgRNA-com-BoxB-vector

    Plasmid

    #132556

    Purpose

    Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.

    Depositor

    Baisong Lu

    Inserts

    dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
    Lambda N22-KRAB
    sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LUC56 (Luc/UGA/SP)

    Plasmid

    #112147

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Photinus pyralis, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258mutated to selenocysteine (U)

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. YFP Gamma 1

    Plasmid

    #36101

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 1 (GNGT1 Bovine)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pGW-FL-V5

    Plasmid

    #78858

    Purpose

    DULIP Prey Destination Vector (Gateway, C-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Firefly luciferase, V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGW-RL-PA

    Plasmid

    #78856

    Purpose

    DULIP Bait Destination Vector (Gateway, C-terminal)

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Renilla luciferase, Protein A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMM545-Tier1-PhCMV-Citrine

    Plasmid

    #169505

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven yellow fluorescent protein citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. [GFP][IRES][Bla]

    Plasmid

    #97194

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge