We narrowed to 9,512 results for: control

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p415GPD-3xHA-GW

Plasmid

#63781

Purpose

yeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insert

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Type

Empty backbone

Tags

3xHA

Expression

Yeast

Promoter

GPD

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2(ΔmScarlet)

Plasmid

#208185

Purpose

GFP positive, mScarlet-I negative control

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Insert

GFPmut2

Use

Reporter

Expression

Bacterial

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJAT57

Plasmid

#204303

Purpose

Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTX1-RNA0

Plasmid

#126040

Purpose

In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

RNA0

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-6-NLS

Plasmid

#50757

Purpose

Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-6 with NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Top-T

Plasmid

#87309

Purpose

Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Top" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-GW

Plasmid

#63780

Purpose

yeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insert

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Type

Empty backbone

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCFP

Plasmid

#175593

Purpose

Lac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.

Depositor

Greg Bokinsky

Article

Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

Insert

CFP (cerulean)

Mutation

An improved monomeric CFP. See doi: 10.1038/nbt945

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJYDN3p

Plasmid

#162457

Purpose

Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSG81-pLas-tetO-deGFPssrA

Plasmid

#102454

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Tags

ssrA

Expression

Bacterial

Promoter

pLas-tetO

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 selectivity (b')

Plasmid

#99837

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (b')

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-122

Plasmid

#171557

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-122

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Bottom-T

Plasmid

#87310

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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PclpB GFP pZa

Plasmid

#170074

Purpose

GFP expression under the control of E. coli clpB promoter

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

PclpB

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pKEW106-MbCas12a-58069

Plasmid

#115653

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR control

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

Plasmid

#75295

Purpose

An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

Depositor

Brandon Harvey

Insert

eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)

Use

AAV

Promoter

human SYN1

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pRL_yEGFP_hc

Plasmid

#98746

Purpose

Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

Insert

Yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Expression

Yeast

Promoter

synthetic

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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AEB-F12

Plasmid

#63849

Purpose

This pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

F12 fluoride riboswitch

Use

Synthetic Biology

Promoter

J23100

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIB empty (w335-1)

Plasmid

#17487

Purpose

Retroviral Empty control vector, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pKEW107-MbCas12a-33362

Plasmid

#115654

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR control

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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