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R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

Plasmid

#159687

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

Depositor

Dominic Esposito

Article

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Insert

His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

Plasmid

#216385

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Mutation

C93A/C95A/C131A

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

Plasmid

#221893

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

TAF15 (TAF15 Human)

Use

Tags

6xHis-MBP-PreScission and TEV-mGFP

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

Plasmid

#221894

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

EWSR1 (EWSR1 Human)

Use

Tags

6xHis-MBP-PreScission and TEV-mGFP

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)

Plasmid

#159546

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV co-transformation cloning vector (13K-C)

Plasmid

#48317

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV co-transformation cloning vector (13S-C)

Plasmid

#48325

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAP

Plasmid

#221896

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

EWSR1 (EWSR1 Human)

Use

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (450-517)-ULK1 (836-1050)-His

Plasmid

#222825

Purpose

Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (363-517)-ULK1 (836-1050)-His

Plasmid

#222824

Purpose

Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

Plasmid

#208872

Purpose

For expression in E. coli of NAP1 (delta-NDP52)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

S37K/A44E (disrupts NDP52 binding)

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

Plasmid

#208873

Purpose

For expression in E. coli of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

Plasmid

#204811

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

Frank von Delft, ASAP Discovery

Insert

A71 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCIneo-V5-SBP-MBP-HsNot4_64-104-GSSG-275-575_AG

Plasmid

#148765

Purpose

Mammalian Expression of HsNot4_64-104-GSSG_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_64-104-GSSG_275-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_109-189-GSSG-275-575_AG

Plasmid

#148767

Purpose

Mammalian Expression of HsNot4_109-189-GSSG_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_109-189-GSSG_275-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP

Plasmid

#190039

Purpose

Expression of FIP200 mutant

Depositor

James Hurley

Article

Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

Insert

FIP200 (RB1CC1 Human)

Use

Tags

GST and MBP

Expression

Mammalian

Mutation

(641-779)deletion & 1363 RDKDLIES 1370 to GSS…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

Plasmid

#177849

Purpose

Bacterial Expression of PNR

Depositor

Karsten Melcher

Article

Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

Insert

photoreceptor cell-specific nuclear receptor (NR2E3 Human)

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r

Plasmid

#162950

Purpose

Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

Plasmid

#162964

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

Plasmid

#162957

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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