59,428 results
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pEGFP-C1-G3BP1-WT
Plasmid#135997PurposeWT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga12
Plasmid#196061PurposeEncodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inserā¦PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga13
Plasmid#196062PurposeEncodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inserā¦PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
TETv4
Plasmid#167983PurposeExpresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoterDepositorInsertTETv4 (TET1CD-XTEN80-dCas9)
UseCRISPR and Synthetic BiologyTags3xNLS, TET1 catalytic domain, and tagBFPExpressionMammalianPromoterCAGAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin WT
Plasmid#12647DepositorAvailable SinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
GSK3betaS9A Ā (1016)
Plasmid#49492Purposeexpresses human Gsk3beta with S9A (constitutively active) mutationDepositorInsertGSK3beta S9A (GSK3B Human)
ExpressionMammalianMutationS9A (constitutively active)PromoterCMVAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin L
Plasmid#11250PurposeMammalian expression of human cathepsin LDepositorHas ServiceCloning Grade DNAInsertcathepsin L (CTSL Human)
TagsC9 (GTETSQVAPA)ExpressionMammalianAvailable SinceMay 25, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-TO-hNIL
Plasmid#172113PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expressionTagsT2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and TRE3GAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Ubiquitin
Plasmid#18712PurposeMammalian expression of human ubiquitin with HA tagDepositorInsertubiquitin (UBB Human)
TagsHAExpressionMammalianMutationThe HA-Ubiquitin insert is from the UBB gene. Theā¦Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
mcherry-PH
Plasmid#36075DepositorAvailable SinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin B
Plasmid#11249PurposeMammalian expression of human cathepsin BDepositorAvailable SinceMay 25, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
JAK2 (V617F)-pcw107-V5
Plasmid#64610Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-1
Plasmid#129726PurposeExpressing SpCas9 and sgAAVS1-1DepositorInsertSpCas9 and sgAAVS1-1
UseCRISPRTagsP2A-PuroExpressionMammalianPromoterhU6Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
ALIX-mNeonGreen
Plasmid#191191PurposeFull-length ALIX with C-terminal mNeonGreen tag.DepositorAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mito-7
Plasmid#55102PurposeLocalization: Mitochondria, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nV5-AMOTL1
Plasmid#172998Purposeconstitutive expression of V5-tagged AMOTL1DepositorAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hMLN
Plasmid#27079PurposeNon-integrating (episomal) expression of human C-MYC, LIN28 and NANOGDepositorInsertC-MYC, LIN28, NANOG (MYC Human)
ExpressionMammalianAvailable SinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV_3XHA-ATP6V1B2-mNeonGreen
Plasmid#228865PurposeStable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreenDepositorAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
