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pLenti-HIFR

Plasmid

#192946

Purpose

Lentiviral production of HIF-1alpha reporter

Depositor

Markus Covert

Article

Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

Insert

HIF-1alpha reporter

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mCMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAMS366-4xCDRE-GFP-PEST

Plasmid

#138658

Purpose

destabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

Depositor

Sabrina Büttner

Article

Diessl et al Microbial Cell, 2020: in press

Insert

4xCDRE-CYC1(PM)-yEGFP3-PEST

Use

Yeast reporter plasmid

Tags

Expression

Mutation

Promoter

CYC1

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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mini-donor - NKX6.1-nEGFP

Plasmid

#206047

Purpose

this mini-donor knockin vector can be used to knock nuclear EGFP into NKX6.1 locus

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

nuclear EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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modRNAc1-Cas9-2A-puro

Plasmid

#216390

Purpose

this vector can be used to make modRNA expressing Cas9 and puro resistance gene

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

Cas9-2A-puro

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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insulator_seq_UENU_donor

Plasmid

#221459

Purpose

Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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4xHRE_RE:RedF

Plasmid

#178314

Purpose

HRE RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

HRE RedFirefly reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-sTomato-NLS-blast

Plasmid

#136259

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

sTomato-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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3xDBE_RE:NLuc

Plasmid

#178318

Purpose

FOXO NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

FOXO NLuc Reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mNeonGreen-NLS-blast

Plasmid

#136258

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mNeonGreen-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mScarletI-NLS-blast

Plasmid

#136260

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mScarletI-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP-V5-B/BRISBANE/60/2008

Plasmid

#187923

Purpose

Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTD103-ARG1

Plasmid

#106475

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_T7-ARG2

Plasmid

#106474

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP-B/BRISBANE/60/2008

Plasmid

#187921

Purpose

Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Use

Tags

UNTAGGED

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a_T5-ARG1

Plasmid

#106476

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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mini-donor - NKX2.5-nEGFP

Plasmid

#209287

Purpose

this mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locus

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

EGFP-nls

Use

Knockin donor vector

Tags

Expression

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTFP-NLS-blast

Plasmid

#136257

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTFP-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mScarletI. PGK-puro

Plasmid

#136266

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mScarletI

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-deltaCKAR

Plasmid

#222429

Purpose

FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Plasma membrane delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-deltaCKAR

Plasmid

#222430

Purpose

FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Nucleus delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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