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Plasmid#70233PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tagDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPA-mCherry-SpHis5
Plasmid#44855PurposeYeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selectionDepositorInsertyoPA-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC11 (pFA6a-TEV-6xGly-V5-KanMX)
Plasmid#44097DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, KanM…ExpressionMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-Kan
Plasmid#44948PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-SpHis5
Plasmid#44853PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GST
Plasmid#41026DepositorInserttetO7-Pcyc1-GST
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-SpHis5
Plasmid#44848PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selectionDepositorInsertyoLSS-mKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA
Plasmid#41024DepositorInserttetO7-Pcyc1-3xHA
UseN-terminal tagging/promoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-LINKER
Plasmid#52685PurposeSame as 41023, with additional linkerDepositorInserttetO7-Pcyc1+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZC10 (pFA6a-PSc-6xGly-V5-His3MX)
Plasmid#44067DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, His3…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC14 (pFA6a-PSc-6xGly-V5-HphMX)
Plasmid#44079DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF260 (pFA6a-PSc-6xGly-KanMX)
Plasmid#44086DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC12 (pFA6a-PSc-6xGly-V5-KanMX)
Plasmid#44091DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, KanM…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Flag-Tipin-L195A
Plasmid#23120DepositorInsertTipin (TIPIN Human)
UseTagsFlag- and HisExpressionInsectMutationChanged Leucine 195 to AlaninePromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST
Plasmid#223764PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
UseTagsThrombin cleavage-GSTExpressionInsectMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-HIS3
Plasmid#229230PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-K.l.LEU2
Plasmid#229229PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-NatMX6
Plasmid#229231PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-TRP1
Plasmid#229228PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only