19,660 results

1. pUG6SP-tTA'

   Plasmid

   #41030

   Depositor

   Michael Nick Boddy

   Article

   Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

   Insert

   tTA' (tetR'-VP16)
   

   Available Since

   Dec. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-HDR-mEGFP-camk2a

   Plasmid

   #104589

   Purpose

   AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Promoter

   None

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmiRFP680-N1

   Plasmid

   #136557

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP680
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. fwYellow chromoprotein

   Plasmid

   #117841

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, fwYellow
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRESneo-FLAG/HA Ago2 corrected

   Plasmid

   #10822

   Depositor

   Thomas Tuschl

   Article

   Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   3 silent mutations in orf

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.3/TO/CD9-GFP

   Plasmid

   #104402

   Purpose

   Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

   Depositor

   Jens Gruber

   Article

   Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

   Insert

   CD9 (CD9 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   CMV

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PL-SIN-EF1α-EGFP

   Plasmid

   #21320

   Purpose

   Control Lentiviral vector with strong EF1a promoter, expresses EGFP

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF5B-FRT-cilia-APEX

   Plasmid

   #73186

   Purpose

   Expresses cilia-APEX in mammalian cells

   Depositor

   Maxence Nachury

   Article

   Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

   Insert

   cilia-APEX (Nphp3 Glycine max, Mouse)
   

   Tags

   internal GFP tag

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-mCh-Cry2olig

   Plasmid

   #101222

   Purpose

   Cry2olig(E490G)

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   Cry2olig
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   SFFV

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEYFP-N1-Pink1

    Plasmid

    #101874

    Purpose

    Transient expression of Pink1-YFP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

    Insert

    PTEN induced putative kinase 1 (PINK1 Human)
    

    Tags

    fluorescent tag EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMTL5

    Plasmid

    #127967

    Purpose

    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. YFP-GID1

    Plasmid

    #37305

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    See comments
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHA-eIF4E

    Plasmid

    #17343

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    eIF4E (EIF4E Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-mCherry-mTrim21

    Plasmid

    #105522

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-VP2SA11

    Plasmid

    #89163

    Purpose

    Express Rotavirus SA11 VP2 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP2
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

    Plasmid

    #132404

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGs-BFP

    Plasmid

    #127348

    Purpose

    Expresses BFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Blue Fluorescent Protein
    

    Expression

    Mammalian

    Promoter

    CAGGS Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120392

    Purpose

    pAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Type

    Empty backbone

    Use

    AAV

    Tags

    mCherry.3XFLAG

    Promoter

    U6

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 acGFP

    Plasmid

    #128047

    Purpose

    Mammalian expression vector with N terminal AcGFP

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    AcGFP

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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