We narrowed to 7,117 results for: 11

1. pDest27-mBIN1

   Plasmid

   #61086

   Purpose

   Express N-terminal GST tagged-mouse BIN1 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   smallest constitutive BIN1excluding exon 7, 11, 13-17
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCAG eGFP-Oct4

   Plasmid

   #203789

   Purpose

   Competitive assay of Oct4 and Oct6 binding to DNA motifs.

   Depositor

   Thorsten Wohland

   Article

   Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1 IntS11 AA 300-597

   Plasmid

   #199329

   Purpose

   Expresses His-tagged Drosophila IntS11 amino acids 300-597

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 11 (IntS11 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 300-597 only

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cry2 mCherry GRK3 ct in pcDNA3.1

   Plasmid

   #64213

   Purpose

   mCherry fused between Cry2 and GRK3 ct to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 PHR (CRY2 Mustard Weed)
   GRK3 ct (GRK3 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

5. myc-POMP

   Plasmid

   #86764

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   human POMP (POMP Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_GNA11_G-alpha

   Plasmid

   #109911

   Purpose

   Protein expression and purification of GNA11_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNA11_G-alpha (GNA11 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-GST-Cys-M87 Spastin

   Plasmid

   #184804

   Purpose

   Bacterial expression of M87 Spastin (A2C mutation)

   Depositor

   James Hurley

   Article

   Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

   Insert

   M87 Spastin (SPAST Human)
   

   Use

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   A2C

   Promoter

   T7

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag‐mTim1 (Bl/6)

   Plasmid

   #49205

   Purpose

   expresses Flag tagged mouse Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (BL/6 allele) (Havcr1 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   Tim1 lacks its own signal sequence

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

9. p426_Cas9_gRNA-ARS1114a

   Plasmid

   #87397

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1114a sequence CTTGTGAAACAAATAATTGG in yeast chromosome 11.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1114a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRevTRE MKL1 S449A/T450A/S454A

    Plasmid

    #19847

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pMSCV-PIG-Lin28b

    Plasmid

    #64795

    Purpose

    Expresses human Lin28b in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Lin28b (LIN28B Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLS3-5xHRE-PHLDA3(wt)

    Plasmid

    #72557

    Purpose

    Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

    Depositor

    Ester Hammond

    Article

    Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

    Insert

    PHLDA3 (PHLDA3 Human)
    

    Use

    Tags

    5XHRE

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDest27-mBIN1+17

    Plasmid

    #61096

    Purpose

    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pRevTRE MKL1-N100 S449A/T450A/S454A

    Plasmid

    #19849

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1-N100 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    deleted amino acids 1-100 and changed Serine 449,…

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

    Plasmid

    #26577

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDN-G1TGt

    Plasmid

    #44508

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV4Neo-murine IL-17RASEFIR-HA

    Plasmid

    #46863

    Purpose

    expresses mouse IL-17RA with SEFIR deletion

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-delta-SEFIR (Il17ra Mouse)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Deletion of SEFIR domain (AA 394-485)

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pGFP-Kif5cmut

    Plasmid

    #71854

    Purpose

    mammalian expression of mutant GFP-Kif5c

    Depositor

    Michelle Peckham

    Article

    Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

    Insert

    Kif5c (Kif5c Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T93N mutation and also a novel SpeI site (1652). …

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pBW_0001

    Plasmid

    #102833

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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