We narrowed to 11,999 results for: SHA

Showing: 6801 - 6820 of 11999 results

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pET15b-Fab1 (Francisella tularensis)

Plasmid

#61690

Purpose

Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

Depositor

Michael Johnson

Article

Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

Insert

enoyl-[acyl-carrier-protein] reductase (NADH)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only
pFN-HaloTag-TalinR8-SpyTag

Plasmid

#192045

Purpose

Plasmid expresses human talin R8 domain flanked between a HaloTag and a SpyTag

Depositor

Ionel Popa

Article

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

TLN1 (TLN1 Human)

Tags

HaloTag and SpyTag

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFN-HaloTag-TalinR7R8-AviTag

Plasmid

#192047

Purpose

Plasmid expresses human talin R7R8 domain flanked between a HaloTag and a AviTag

Depositor

Ionel Popa

Article

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

TLN1 (TLN1 Human)

Tags

AviTag and HaloTag

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.13.EFS-NS.H2B-RFP

Plasmid

#170388

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a SVEM hphMX6 lys1-I-SceI cleavage site

Plasmid

#67648

Purpose

I-SceI cleavage site at lys1-hphMX6

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

lys1 (lys1 Fission Yeast)
hphMX6

Use

To test i-scei cleavage efficiency

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS315-48nt-telo-I-SceI

Plasmid

#67623

Purpose

telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
48 bp telomere repeats
hph

Use

Yeast cen/ars plasmid

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 cyto Grx1-roGFP2

Plasmid

#64999

Purpose

Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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PGEX-DDX21-D2+C

Plasmid

#179243

Purpose

protein purification in E. coli

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21-D2+C (DDX21 Human)

Tags

GST-flag

Expression

Bacterial

Mutation

aa396-782

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-GST

Plasmid

#39287

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 HRPT2 L64P

Plasmid

#11052

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 L64P (CDC73 Human)

Tags

Flag

Expression

Mammalian

Mutation

L64P

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pVLAD6-mmPtch1-B-SBP

Plasmid

#120886

Purpose

BacMam vector for Ptch1-B expression

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

SBP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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R9-WT

Plasmid

#125210

Purpose

Expressing wildtype human Rad9b gene

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Expression

Mammalian

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA INPP5E KD1

Plasmid

#162004

Purpose

INPP5E shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5E (INPP5E Canis Lupus)

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159673

Purpose

Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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hC73A-pEGFP_N1

Plasmid

#111533

Purpose

Expresses human KCNB1 C73A in mammalian cells for imaging

Depositor

Federico Sesti

Article

Yu et al Cell Death Dis. 2017 Apr 6;8(4):e2737. d

Insert

human C73A-Kv2.1 (KCNB1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C73A

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-HA-Tbkbp1

Plasmid

#125177

Purpose

Retroviral expression of mouse Tbkbp1

Depositor

Shao-Cong Sun

Article

Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

Insert

Tbkbp1 (Tbkbp1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-ATXN1L

Plasmid

#16167

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Similar to Ataxin1 (ATXN1L Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L57E

Plasmid

#47337

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L57E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L74E

Plasmid

#47338

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

Plasmid

#82469

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mRNA capping enzyme (Rngtt Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

changed Lysine 294 to Alanine

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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