We narrowed to 17,863 results for: STO;

1. pET-28a-His6-SUMO-VAMP8

   Plasmid

   #135553

   Purpose

   Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP8
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG HDAC5 S498A

   Plasmid

   #32215

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser498Ala

   Promoter

   CMV

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

3. BB1_34_RPS25Att

   Plasmid

   #98526

   Purpose

   BB1 with insert: RPS25Att

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   RPS25Att
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

   Plasmid

   #168169

   Purpose

   Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
   

   Tags

   YFP

   Expression

   Worm

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 FYN

   Plasmid

   #67106

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   FYN (FYN Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. p63RhoGEF580-mVenus-N1

   Plasmid

   #84338

   Purpose

   Expresses p63 (580 a.a.) tagged with mVenus

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

   Insert

   p63RhoGEF (580 aa) (ARHGEF25 Human)
   

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mPapaya-H2B-C-10

   Plasmid

   #56396

   Purpose

   Localization: Nucleus/Histones, Excitation: 530, Emission: 541

   Depositor

   Michael Davidson

   Article

   Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

   Insert

   H2B (H2BC11 Human)
   

   Tags

   mPapaya

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G03 CREBZF

   Plasmid

   #67070

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CREBZF (CREBZF Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3_PKA-CαN(E12,14K)-mPAGFP

   Plasmid

   #168495

   Purpose

   Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

   Insert

   E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
   

   Expression

   Mammalian

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 RL3Am

    Plasmid

    #109364

    Purpose

    Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Branchiostoma belcheri, Human)
    

    Tags

    1D4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHL 651

    Plasmid

    #53016

    Purpose

    LacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    LacIq
    tetR
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and T1 terminator

    Expression

    Bacterial

    Promoter

    pCon

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pSG5-Myr-FLAG-p110β-DM

    Plasmid

    #55719

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 beta
    

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    S205D, K224A

    Promoter

    T7

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pACYC-OspCas12c-noncoding

    Plasmid

    #120878

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-OspCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB3043(gRNA XI-1)

    Plasmid

    #73285

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-bSV1_1-1009

    Plasmid

    #129471

    Purpose

    Mammalian expression of the N-terminus + central domain of bovine SV1

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    Supervillin (SVIL Bovine)
    

    Expression

    Mammalian

    Mutation

    contains amino acids 1-1009

    Promoter

    CMV

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHL 1277

    Plasmid

    #53017

    Purpose

    AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Inserts

    GFP
    mCherry
    

    Use

    Synthetic Biology

    Tags

    RBS(st7)

    Expression

    Bacterial

    Promoter

    pLlacO-1 and pLtetO-1

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCfB3049(gRNA XII-4)

    Plasmid

    #73291

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. STAGR_SAMScaffold_h7SK

    Plasmid

    #102846

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_h7SK
    

    Use

    Pcr template for stagr inserts

    Promoter

    H7SK

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCellFree_G03 CSNK2A1

    Plasmid

    #67085

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. JM3 [JBEI-6891]

    Plasmid

    #89788

    Purpose

    Broad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoter

    Depositor

    Harry Beller

    Article

    Muller et al Appl Environ Microbiol. 2013 Jul;79(14):

    Insert

    tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
    

    Expression

    Bacterial

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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